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Team:UiOslo Norway/Notebook
From 2014.igem.org
Notebook
Welcome to our notebook! Here you can see what we've done each week in the lab, in media and everything else. Click below to navigate between weeks.
Week 18
Thursday
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- This
- And that
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Friday
Nothing much yo.
Week 19
Thursday
Lorem ipsum dolor sit amet, consectetur adipisicing elit. Dolor, eos.
- This
- And that
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This text is bold because of the strong tag
Friday
Nothing much yo.
Week 20
Friday
Nothing much yo.
Week 21
Friday
Nothing much yo.
Week 22
Friday
Nothing much yo.
Week 23
Friday
Nothing much yo.
Week 24
Thursday William
PCR on BL21 and AB2848 strain on bacteria to retrieve LacZ-beta gene. Used LacZ-beta STOP primer.
Agarose gel electrophoresis result: Band about 3 kb for the BL21 strain. Probably LacZ-beta.
Friday William
PCR clean-up using Machery-Nagel PCR clean-up kit. Followed protocol from the kit.
PCR amplification of the cleaned DNA with and without stop codon.
Week 25
Tuesday
PCR clean-up using Machery-Nagel PCR clean-up kit on DNA with and without stop codon.
We did step 1 of making E. coli TOP10 cells competent (incubation of cells from freeze stock in LB medium with streptomycin.
We analyzed the DNA concentration using NanoDrop:
LacZ-beta with stop codon: 230 ng/µl
LacZ-beta without stop codon: 300 ng/µl
Week 26
Friday
Nothing much yo.
Week 27
Friday
Nothing much yo.
Week 28
Friday
Nothing much yo.
Week 29
Friday
Nothing much yo.
Week 30
Friday
Nothing much yo.
Week 31
Friday
Nothing much yo.
Week 32
Friday
Nothing much yo.
Week 33
Friday
Nothing much yo.
Week 34
Friday
Nothing much yo.
Week 35
Friday
Nothing much yo.
Week 36
Friday
Nothing much yo.
Week 37
Friday
Nothing much yo.
Week 38
Friday
Nothing much yo.
Week 39
Friday
Nothing much yo.
Week 40
Friday
Nothing much yo.
Week 41
Friday
Nothing much yo.
Week 42
Friday
Nothing much yo.
Week 43
Friday
Nothing much yo.
Week 44
Friday
Nothing much yo.
