Team:SCUT

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<h1 id="ProjectName">Aromatics Scouts<img id="LittleScout" src="https://static.igem.org/mediawiki/igem.org/8/8a/Peking2013_home_Telescope.png" /></h1>
 
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                <h1 id="ProjectSubname">A Comprehensive Biosensor Toolkit for Profiling Aromatic Compounds in the Environment</h1>
 
   
   
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<B>★Presentation:</B> Room 34-101, Saturday 12:30 PM, Session 2
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      &ensp;&ensp;&ensp;&ensp;<B>★Poster:</B> Sunday, #13 (Stata)
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              <p id="AbstractContent">Monitoring aromatic compounds in the environment remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a functionally comprehensive biosensor toolkit to profile aromatics in the environment.
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<br/><br/>
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Transcriptional regulators that each detect a specific class of aromatics were first bioinformatically determined; and then utilized to build a comprehensive set of biosensor circuits. Characterization on the detection profiles of individual biosensors and the orthogonality/crosstalk between them proved that these biosensors are very capable at profiling aromatics present in water.
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<br/><br/>
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Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable compounds into detectable compounds, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.
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<br/><br/>
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We expect that these novel biosensors, together with the plug-in devices, will serve as intriguing synthetic biological tools for diverse practical applications.
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        <div id="AppendixBox1" class="SmallBoxes" >
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              <h1 id="Appendix1Title"><i>Mining aromatics-sensing Biobricks <br/>from the genomic database</i></h1>
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                    <h1 class="AppendixHeadLine">Biosensor Mining</h1>
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                    <p>The core component of our biosensor toolkit is the transcriptional regulators that sense aromatics. For the comprehensiveness of aromatics-sensing, a data-mining process was conducted to mine transcriptional regulators for each typical class of aromatics from the database Uniprot. </p>
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                    <a href="https://2013.igem.org/Team:Peking/Project/SensorMining">LEARN MORE</a>
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              <h1 id="Appendix2Title"><i>High-performance, profile-<br/>specific biosensors</i> </h1>
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                    <h1 class="AppendixHeadLine">Biosensors</h1>
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                    <p>A comprehensive set of biosensor circuits have been implemented using the aromatics-sensing transcriptional regulators. Each biosensor has a specific aromatics-sensing profile. Furthermore, the orthogonality of their sensing profiles was carefully assessed for practical applications.</p>
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                    <a href="https://2013.igem.org/Team:Peking/Project/BioSensors">LEARN MORE</a>
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              <h1 id="Appendix3Title"><i>Converting the undetectable into<br/>the detectable</i></h1>
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                    <h1 class="AppendixHeadLine">Adaptors</h1>
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                    <p> To expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were taken from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits.</p>
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                    <a href="https://2013.igem.org/Team:Peking/Project/Plugins">LEARN MORE</a>
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              <h1 id="Appendix4Title"><i>Rapidly determining <br/> the analyte concentration</i></h1>
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                    <h1 class="AppendixHeadLine">Band-pass Filter</h1>
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                    <p>For the ease of practical analysis, a genetic device called "Band-pass Filter" has been constructed to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter can robustly quantify the aromatics in environmental samples. </p>
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                    <a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">LEARN MORE</a>
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              <h1 id="Appendix5Title"><i>Communications, Questionnaire survey,  <br/>Factory Visit</i> and interview</h1>
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                    <p>To obtain the information about public awareness and the situation of aromatics pollution, a survey including factory visit and questionnaires has been conducted. We also guided an iGEM HS team and held "Model iGEM" as a competition without competitiveness.</p>
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                    <a href="https://2013.igem.org/Team:Peking/HumanPractice">LEARN MORE</a>
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Latest revision as of 13:59, 16 November 2014

Retrieved from "http://2014.igem.org/Team:SCUT"