"
Team:LZU-China/tempfortest
From 2014.igem.org
(Difference between revisions)
| (9 intermediate revisions not shown) | |||
| Line 3: | Line 3: | ||
<head> | <head> | ||
<meta http-equiv="Content-Type" content="text/html; charset=gb2312" /> | <meta http-equiv="Content-Type" content="text/html; charset=gb2312" /> | ||
| - | <title> | + | <title>LZU-China 2014</title> |
<style type="text/css"> | <style type="text/css"> | ||
| Line 216: | Line 216: | ||
@charset "utf-8"; | @charset "utf-8"; | ||
| - | body,h1,h2,h3,h4,h5,h6,hr,p,blockquote,dl,dt,dd,ul,ol,li,pre,form,fieldset,legend,button,input,textarea,th,td,figure{margin:0;padding:0} | + | body,h1,h2,h3,h4,h5,h6,hr,p,blockquote,dl,dt,dd,ul,ol,li,pre,form,fieldset,legend,button,input,textarea,th,td,figure{ |
| + | margin:0; | ||
| + | padding:0; | ||
| + | font-size: 14px; | ||
| + | text-align: left; | ||
| + | } | ||
html{background-color:#e9ecec} | html{background-color:#e9ecec} | ||
table{table-layout:fixed;border-collapse:collapse} | table{table-layout:fixed;border-collapse:collapse} | ||
| Line 230: | Line 235: | ||
sub{vertical-align:text-bottom} | sub{vertical-align:text-bottom} | ||
legend{color:#000} | legend{color:#000} | ||
| - | fieldset,img{border:0 none} | + | fieldset,img{ |
| + | border:0 none; | ||
| + | font-size: 15px; | ||
| + | } | ||
button,input,select,textarea{font-size:100%} | button,input,select,textarea{font-size:100%} | ||
| - | table{border-collapse:collapse;border-spacing:0} | + | table{ |
| + | border-collapse:collapse; | ||
| + | border-spacing:0; | ||
| + | text-align: center; | ||
| + | } | ||
input:focus{outline:none} | input:focus{outline:none} | ||
.hd-main{height:60px;background:#fff;z-index:15;border-bottom:#b3b3b3 1px solid;box-shadow:0 0 5px #333} | .hd-main{height:60px;background:#fff;z-index:15;border-bottom:#b3b3b3 1px solid;box-shadow:0 0 5px #333} | ||
| Line 239: | Line 251: | ||
.hd-main .has-pulldown{cursor:pointer;position:relative;*z-index:10000} | .hd-main .has-pulldown{cursor:pointer;position:relative;*z-index:10000} | ||
.hd-main .pulldown{position:absolute;cursor:default;display:none;top:30px;left:0} | .hd-main .pulldown{position:absolute;cursor:default;display:none;top:30px;left:0} | ||
| - | .hd-main .pulldown .arrow{*margin-bottom:-1px;_margin-bottom:0;height:0;width:0;font-size:0;line-height:0;border-width:7px;border-style:solid;border-color:transparent transparent rgba(255, 255, 255,0.8) transparent;_filter:chroma(color=tomato);_border-color:tomato tomato rgba(255, 255, 255,0.8) tomato;position:relative;display:block;left:20px;z-index: | + | .hd-main .pulldown .arrow{*margin-bottom:-1px;_margin-bottom:0;height:0;width:0;font-size:0;line-height:0;border-width:7px;border-style:solid;border-color:transparent transparent rgba(255, 255, 255,0.8) transparent;_filter:chroma(color=tomato);_border-color:tomato tomato rgba(255, 255, 255,0.8) tomato;position:relative;display:block;left:20px;z-index:7} |
| - | .hd-main .pulldown .content{background:rgba(255, 255, 255,0.8);color:#333;text-align:left;border-radius:3px;border:rgb(175,175,175) 1px solid;border-width:0 1px 1px 1px;box-shadow:0 2px 4px rgba(0,0,0,.2);position:relative;z-index: | + | .hd-main .pulldown .content{background:rgba(255, 255, 255,0.8);color:#333;text-align:left;border-radius:3px;border:rgb(175,175,175) 1px solid;border-width:0 1px 1px 1px;box-shadow:0 2px 4px rgba(0,0,0,.2);position:relative;z-index:6} |
.hd-main .pulldown-canvas{position:absolute;top:0;left:0;width:100%;height:100%;opacity:0;filter:alpha(opacity=0)} | .hd-main .pulldown-canvas{position:absolute;top:0;left:0;width:100%;height:100%;opacity:0;filter:alpha(opacity=0)} | ||
.hd-main .logo{background-image:url('https://static.igem.org/mediawiki/2014/7/72/2014logomin.png');background-repeat:no-repeat;display:block;height:36px;width:175px;float:left;margin-left:10px;background-position:0 0;margin-top:5px;display:inline} | .hd-main .logo{background-image:url('https://static.igem.org/mediawiki/2014/7/72/2014logomin.png');background-repeat:no-repeat;display:block;height:36px;width:175px;float:left;margin-left:10px;background-position:0 0;margin-top:5px;display:inline} | ||
| Line 247: | Line 259: | ||
.hd-main .navs a:hover,.hd-main .navs a:active{text-decoration:none;background-color:#0f0f0f} | .hd-main .navs a:hover,.hd-main .navs a:active{text-decoration:none;background-color:#0f0f0f} | ||
.hd-main .navs .def-nav,.hd-main .navs .pulldown-nav,.hd-main .navs .separate{display:block;float:left;height:48px;font:18px/30px "Microsoft YaHei","Microsoft JhengHei";color:#d8d8d8;text-align:center;width:125px;line-height:48px} | .hd-main .navs .def-nav,.hd-main .navs .pulldown-nav,.hd-main .navs .separate{display:block;float:left;height:48px;font:18px/30px "Microsoft YaHei","Microsoft JhengHei";color:#d8d8d8;text-align:center;width:125px;line-height:48px} | ||
| - | .hd-main .navs .def-nav .app-url{left:185px;width: | + | .hd-main .navs .def-nav .app-url{left:185px;width:425px;top:48px} |
| - | .hd-main .navs .def-nav .pulldown-canvas-url{width: | + | .hd-main .navs .def-nav .pulldown-canvas-url{width:575px;height:109px} |
.hd-main .navs .def-nav .app-url .arrow{left:140px} | .hd-main .navs .def-nav .app-url .arrow{left:140px} | ||
.hd-main .navs .app-url .content{padding:17px 0 0 22px;height:91px} | .hd-main .navs .app-url .content{padding:17px 0 0 22px;height:91px} | ||
| Line 314: | Line 326: | ||
} | } | ||
/*<
In wet lab, we designed a novel MFC device containing genetic engineered bacteria. For anode strain, we’ve cloned a PNP sensor sequence and a riboflavin into Escherichia coli. The recombinants is able to detect PNP and produce riboflavin to boost electrical generation when co-cultured with Shewanella oneidensis. |
|
 |
|
We found that the part BBa_K381001 by iGEM10_BCCS-Bristol is a sensor which can detect the appearance of nitrate and nitrite. So we perfomed an experience by this part to see if this part can detect PNP(p-Nitrophenol). We got a good result.You can see the PNP can also induce the green fluorescence.
Figure-1 The pathway of PNP sensor
Figure-1 The pathway of PNP sensor
Figure-1 The pathway of PNP sensor
|
|
|
|
We found four genes to reduce the Cr(VI) to Cr(III). They are nahG, nahR, nahE, chrR and yieF. We didn’t do the deep research about them because of time limitation. They can be used in the general plasmids such as pBR322. We just made them to be the formation of Biobrick. More uses and functions need to be demonstrated and found. nahG: We got the sequence from Pseudomonas putida (Primer:sense:5’---CCGGAATTCGCGGCCGCTTCTAGATGAAAAACAATAAACCTGGCTTGCGC---3’;
(Primer:sense:5’--- CCGGAATTCGCGGCCGCTTCTAGATGAAAAAAATAGTCCAGTCGGAAGG---3’;
(Primer:sense:5’--- CCGGAATTCGCGGCCGCTTCTAGATGAAAAAAATAGTCCAGTCGGAAGG---3’;
|
