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Team:LZU-China
From 2014.igem.org
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<meta http-equiv="Content-Type" content="text/html; charset=gb2312" /> | <meta http-equiv="Content-Type" content="text/html; charset=gb2312" /> | ||
| - | <title> | + | <title>LZU-China 2014</title> |
<style type="text/css"> | <style type="text/css"> | ||
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#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {display: none;} /*-- hides default wiki settings --*/ | #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {display: none;} /*-- hides default wiki settings --*/ | ||
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.annItem li {list-style: none; margin: 5px} | .annItem li {list-style: none; margin: 5px} | ||
| - | + | @charset "utf-8"; | |
| - | + | body,h1,h2,h3,h4,h5,h6,hr,p,blockquote,dl,dt,dd,ul,ol,li,pre,form,fieldset,legend,button,input,textarea,th,td,figure{ | |
| - | + | margin:0; | |
| - | + | padding:0; | |
| - | + | font-size: 14px; | |
| - | + | text-align: left; | |
| - | + | ||
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} | } | ||
| - | # | + | html{background-color:#e9ecec} |
| + | table{table-layout:fixed;border-collapse:collapse} | ||
| + | section,article,aside,header,footer,nav,dialog,figure{display:block} | ||
| + | .clearfix:after{content:"\20";display:block;height:0;line-height:0;font-size:0;clear:both} | ||
| + | body,button,input,select,textarea,td,th{color:#333;font:12px/1.5 tahoma,arial,\5b8b\4f53} | ||
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| + | sup{vertical-align:text-top} | ||
| + | sub{vertical-align:text-bottom} | ||
| + | legend{color:#000} | ||
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| + | button,input,select,textarea{font-size:100%} | ||
| + | table{ | ||
| + | border-collapse:collapse; | ||
| + | border-spacing:0; | ||
| + | text-align: center; | ||
} | } | ||
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| + | .hd-main{height:60px;background:#fff;z-index:15;border-bottom:#b3b3b3 1px solid;box-shadow:0 0 5px #333} | ||
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| + | .hd-main{padding:0 5px;height:49px;min-width:800px;background:#252525;border-bottom:0} | ||
| + | .hd-main .has-pulldown{cursor:pointer;position:relative;*z-index:10000} | ||
| + | .hd-main .pulldown{position:absolute;cursor:default;display:none;top:30px;left:0} | ||
| + | .hd-main .pulldown .arrow{*margin-bottom:-1px;_margin-bottom:0;height:0;width:0;font-size:0;line-height:0;border-width:7px;border-style:solid;border-color:transparent transparent rgba(255, 255, 255,0.8) transparent;_filter:chroma(color=tomato);_border-color:tomato tomato rgba(255, 255, 255,0.8) tomato;position:relative;display:block;left:20px;z-index:7} | ||
| + | .hd-main .pulldown .content{background:rgba(255, 255, 255,0.8);color:#333;text-align:left;border-radius:3px;border:rgb(175,175,175) 1px solid;border-width:0 1px 1px 1px;box-shadow:0 2px 4px rgba(0,0,0,.2);position:relative;z-index:6} | ||
| + | .hd-main .pulldown-canvas{position:absolute;top:0;left:0;width:100%;height:100%;opacity:0;filter:alpha(opacity=0)} | ||
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| + | .hd-main .navs{padding:0 0 3px 25px;height:45px;float:left} | ||
| + | .hd-main .navs a,.hd-main .info a,.hd-main .navs a:visited,.hd-main .info a:visited,.hd-main .navs a:active,.hd-main .info a:active{color:#d8d8d8} | ||
| + | .hd-main .navs a:hover,.hd-main .navs a:active{text-decoration:none;background-color:#0f0f0f} | ||
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| + | .hd-main .navs .def-nav .app-url{left:185px;width:425px;top:48px} | ||
| + | .hd-main .navs .def-nav .pulldown-canvas-url{width:575px;height:109px} | ||
| + | .hd-main .navs .def-nav .app-url .arrow{left:140px} | ||
| + | .hd-main .navs .app-url .content{padding:17px 0 0 22px;height:91px} | ||
| + | .hd-main .navs .app-url .content a,.hd-main .navs .app-url .content a:hover{color:#333} | ||
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| + | .hd-main .navs .app-url .li:hover{background-color:#e0e1e5} | ||
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| + | .hd-main .navs .app-url .li:hover .icon{background-image:url('https://static.igem.org/mediawiki/2014/9/94/Icons50X50nb.png')} | ||
| + | .hd-main .navs .app-url .tongxunlu .icon{background-position:-50px 0} | ||
| + | .hd-main .navs .app-url .xiangce .icon{background-position:-100px 0} | ||
| + | .hd-main .navs .app-url .note .icon{background-position:-150px 0} | ||
| + | .hd-main .navs .app-url .duanxin .icon{background-position:-200px 0} | ||
| + | .hd-main .navs .app-url .zhaohui .icon{background-position:-250px 0} | ||
| + | .hd-main .navs .app-url .youxi .icon{background-position:-300px 0} | ||
| + | .hd-main .navs .app-url .li .text{height:30px;display:block;font:10px/30px "Microsoft YaHei","Microsoft JhengHei"} | ||
| + | .hd-main .navs .separate{margin:0;height:48px;width:2px;background:#2A2B2D} | ||
| + | .hd-main .navs .pulldown-nav{position:relative} | ||
| + | .hd-main .navs .pulldown-nav em{ | ||
| + | position:absolute; | ||
| + | display:block; | ||
| + | left:99px; | ||
| + | top:18px; | ||
| + | height:12px; | ||
| + | width:12px; | ||
| + | font-size:0; | ||
| + | background-position:-89px -45px | ||
} | } | ||
| - | # | + | .hd-main .navs .pulldown-nav:hover .f-icon,.hd-main .navs .pulldown-nav:active .f-icon{position:absolute;display:block;right:11px;top:18px;height:12px;width:12px;font-size:0;background-position:-89px -55px} |
| + | .hd-main .navs .current,.hd-main .navs .current .pulldown-nav{font-weight:bold;color:#FFF;display:inline-block;width:125px;height:49px;background-color:#0f0f0f;text-align:center;line-height:48px} | ||
| + | .hd-main .navs .current:hover{color:#ECECEC} | ||
| + | .hd-main .navs .current .hover{background-color:#dedede;color:#333;} | ||
| + | .hd-main .info,.hd-main .info ul{height:36px;float:right;text-align:right;margin-top:3px;white-space:nowrap} | ||
| + | .hd-main .info a{color:#d8d8d8} | ||
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| + | .hd-main{padding:0 5px;height:49px;min-width:800px;background:#252525;border-bottom:0} | ||
| + | .hd-main .info .has-pulldown{padding-right:28px} | ||
| + | .hd-main .info .has-pulldown .pull-arrow{position:absolute;display:block;right:10px;top:1px;height:12px;width:12px;font-size:0;background-position:-89px -45px} | ||
| + | .hd-main .info .no-separate{border:none} | ||
| + | .hd-main .info .user-name{position:relative;padding-left:24px} | ||
| + | .hd-main .info .user-name .name{display:inline-block;display:inline\0;white-space:nowrap;max-width:75px;overflow:hidden;text-overflow:ellipsis;vertical-align:bottom} | ||
| + | .hd-main .info .user-name .identity{position:absolute;top:0;left:0;height:16px;width:16px;background-position:-89px -88px} | ||
| + | .hd-main .info .user-name .is-vip{background-position:-89px -68px} | ||
| + | .hd-main .info .user-info,.hd-main .info .more-info{top:9px;left:auto;right:-24px;width:170px} /*User栏目宽度*/ | ||
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| + | .hd-main .info .notice-nu .f-icon{display:inline-block;padding-right:4px;background-position:right -109px;color:#FFF} /*此处原为#FFF*/ | ||
| + | .hd-main .info .user-info .arrow{left:70px;*left:-32px} | ||
| + | .hd-main .info .user-info .content,.hd-main .info .more-info .content{padding:10px 0;height:180px} | ||
| + | .hd-main .info .more{padding:0;display:inline;line-height:inherit;background:none} | ||
| + | .hd-main .info .more-info .content{height:150px} | ||
| + | .hd-main .info .user-info .content a,.hd-main .info .more-info .content a{display:block;padding-left:33px;height:30px;line-height:30px;color:#888} | ||
| + | .hd-main .info .user-info .content a:hover,.hd-main .info .more-info .content a:hover{color:#333;text-decoration:none;background:#e0e1e5} | ||
| + | .hd-main .info .user-info .li,.hd-main .info .more-info .li{display:block;padding:2px 0;height:30px} | ||
| + | .hd-main .info .user-info .separate-li,.hd-main .info .more-info .separate-li{display:block;margin:3px 11px;border-top:#EBEBEC 1px solid;border-bottom:#FFF 1px solid} | ||
| + | .hd-main .info .more-info{right:0} | ||
| + | .hd-main .info .more-info .arrow{left:94px;*left:-8px} | ||
| + | #apDiv1 { | ||
position:absolute; | position:absolute; | ||
| - | left: | + | left:3px; |
| - | top: | + | top:4px; |
| - | width: | + | width:100%; |
| - | height: | + | height:900px; |
| - | z-index: | + | z-index:0; |
| - | + | background-color: #66CCFF; | |
| - | + | ||
| - | + | ||
} | } | ||
| + | /*<![CDATA[*/ | ||
| + | body { | ||
| + | margin:0; | ||
| + | padding:0 10px 0 10px; | ||
| + | border:0; | ||
| + | height:100%; | ||
| + | overflow:auto; | ||
| + | background-color: #FFF; | ||
| + | } | ||
| + | #menu {right:0; top:0; width:100%; position:fixed; padding:10px; text-align:center; font-weight:bold; background:rgba(0,83,163,0);} | ||
| + | * html #menu {position:absolute; right:16px; top:0;}/*only for ie*/ | ||
| + | html {overflow:auto !important; overflow:hidden;} | ||
| + | |||
#apDiv2 { | #apDiv2 { | ||
position:absolute; | position:absolute; | ||
| - | left: | + | left:0; |
| - | top: | + | top:22px; |
| - | width: | + | width:100%; |
| - | height: | + | height:541px; |
| - | z-index: | + | z-index:0; |
| - | color: # | + | background-color: #187BDB; |
| - | + | ||
| - | + | ||
| - | + | ||
} | } | ||
| - | # | + | #apDiv2b{ |
position:absolute; | position:absolute; | ||
| - | left: | + | left:0; |
| - | top: | + | top:2400px; |
| - | width: | + | width:100%; |
| - | height: | + | height:1024px; |
z-index:1; | z-index:1; | ||
| - | + | background-color: #187BDB; | |
} | } | ||
| - | # | + | #apDiv3 { |
position:absolute; | position:absolute; | ||
left:1px; | left:1px; | ||
| - | top: | + | top:570px; |
| - | width: | + | width:100%; |
| - | height: | + | height:150px; |
| - | z-index: | + | z-index:0; |
| - | + | ||
| - | + | ||
} | } | ||
| - | # | + | #apDiv3 table tr td p { |
| - | + | font-size: 15px; | |
| - | + | text-align: center; | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
} | } | ||
| - | # | + | #apDiv4 { |
position:absolute; | position:absolute; | ||
left:0; | left:0; | ||
| - | top: | + | top:1224px; |
| - | width: | + | width:100%; |
| - | height: | + | height:56px; |
z-index:0; | z-index:0; | ||
| - | |||
| - | |||
| - | |||
| - | |||
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} | } | ||
| - | # | + | #apDiv5 { |
position:absolute; | position:absolute; | ||
| - | left: | + | left:0; |
| - | top: | + | top:3118px; |
| - | width: | + | width:100%; |
| - | height: | + | height:25; |
| - | z-index: | + | z-index:2; |
| - | + | background-color: #5FC1C5; | |
} | } | ||
| - | # | + | #apDiv3 div { |
| + | font-size: 46px; | ||
text-align: center; | text-align: center; | ||
} | } | ||
| - | # | + | #apDiv4 table tr td div { |
| + | font-size: 45px; | ||
| + | color: #000; | ||
| + | text-align: left; | ||
| + | } | ||
| + | #apDiv4 table tr td table tr td { | ||
| + | font-size: 16px; | ||
| + | line-height: 30px; | ||
| + | text-align: left; | ||
| + | } | ||
| + | body,td,th { | ||
| + | color: #333; | ||
| + | } | ||
| + | #apDiv6 { | ||
position:absolute; | position:absolute; | ||
| - | left: | + | left:575px; |
| - | top: | + | top:3007px; |
| - | width: | + | width:220px; |
| - | height: | + | height:221px; |
z-index:3; | z-index:3; | ||
| - | |||
} | } | ||
| - | # | + | #apDiv3 table tr td div { |
| - | + | font-size: 19px; | |
| - | + | color: #666; | |
| - | + | font-weight: bold; | |
| - | + | } | |
| - | + | #apDiv4 table tr td table tr td table tr td { | |
| - | + | text-align: center; | |
| + | } | ||
| + | #apDiv4 table tr td table tr td p { | ||
| + | font-size: 16px; | ||
| + | line-height: 31px; | ||
} | } | ||
</style> | </style> | ||
| - | </ | + | </head> |
| - | < | + | <body id="" class=""> |
| - | <div id=" | + | <div id="apDiv2"> |
| - | < | + | <p><img src="lzuchianmfctt.jpg" width="1366" height="541" /></p> |
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | </div> | ||
| + | |||
| + | <body id="" class=""> | ||
| + | <div id="apDiv2b"> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2014/b/bc/Lzumfctt2.jpg" width="1366" height="542" /></p> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2014/4/42/Bkcolorind.jpg" width="1366" height="482" /></p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <script type="text/javascript"> | ||
| + | var _bdhmProtocol = (("https:" == document.location.protocol) ? " https://" : " http://"); | ||
| + | document.write(unescape("%3Cscript src='" + _bdhmProtocol + "hm.baidu.com/h.js%3F986011a32f84f432107ce07502765e79' type='text/javascript'%3E%3C/script%3E")); | ||
| + | </script> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | </div> | ||
| + | <div id="apDiv2"> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2014/c/c6/Lzuchianmfctt.jpg" width="1366" height="541" /></p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | |||
| + | <div id="apDiv3"> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <div>CONTENTS</div> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <table width="100%" border="0" align="center"> | ||
<tr> | <tr> | ||
| - | <td | + | <td width="20%"><p> </p> |
| + | <p> </p></td> | ||
| + | <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/background"><img src="https://static.igem.org/mediawiki/2014/8/83/1backgroud.png" width="100" height="100" /></a></p> | ||
| + | <p>Background</p></td> | ||
| + | <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/wetlab"><img src="https://static.igem.org/mediawiki/2014/d/d5/2wetlab.png" width="100" height="100" /></a></p> | ||
| + | <p>Wet Lab</p></td> | ||
| + | <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/drylab"><img src="https://static.igem.org/mediawiki/2014/c/c6/3drylab.png" width="100" height="100" /></a></p> | ||
| + | <p>Dry Lab</p></td> | ||
| + | <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/Parts"><img src="https://static.igem.org/mediawiki/2014/1/14/4parts.png" width="100" height="100" /></a></p> | ||
| + | <p>Parts</p></td> | ||
| + | <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/hp"><img src="https://static.igem.org/mediawiki/2014/5/5f/5humanpractics.png" width="100" height="100" /></a></p> | ||
| + | <p>Human Practice</p></td> | ||
| + | <td width="20%"> </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>< | + | <td width="20%"> </td> |
| - | <td><a href="https://2014.igem.org/Team:LZU-China"><img src="https://static.igem.org/mediawiki/2014/ | + | <td width="12%"><p> </p> |
| - | <td><img src="https://static.igem.org/mediawiki/2014/c/ | + | <p> </p> |
| - | <td><img src="https://static.igem.org/mediawiki/2014/ | + | <p> </p></td> |
| - | <td><img src="https://static.igem.org/mediawiki/2014/ | + | <td width="12%"> </td> |
| - | <td><img src="https://static.igem.org/mediawiki/2014/ | + | <td width="12%"> </td> |
| + | <td width="12%"><p> </p></td> | ||
| + | <td width="12%"> </td> | ||
| + | <td width="20%"> </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td width="20%"> </td> | ||
| + | <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/Team"><img src="https://static.igem.org/mediawiki/2014/a/a2/6members.png" width="100" height="100" /></a></p> | ||
| + | <p>Our team</p></td> | ||
| + | <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/interlab"><img src="https://static.igem.org/mediawiki/2014/c/cc/7interlab.png" width="100" height="100" /></a></p> | ||
| + | <p>Interlab</p></td> | ||
| + | <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/Safety"><img src="https://static.igem.org/mediawiki/2014/e/ee/8safety.png" width="100" height="100" /></a></p> | ||
| + | <p>Safety</p></td> | ||
| + | <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/Notebook"><img src="https://static.igem.org/mediawiki/2014/0/00/9notebook.png" width="100" height="100" /></a></p> | ||
| + | <p>Notebook</p></td> | ||
| + | <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/future"><img src="https://static.igem.org/mediawiki/2014/0/04/10future.png" width="100" height="100" /></a></p> | ||
| + | <p>Future Work</p></td> | ||
| + | <td width="20%"> </td> | ||
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| - | < | + | <td><div> *In order to get better user experience, we recommend the Internet Explorer 10 (or higher version). And the <span id="tran_1_0" data-aligning="#src_1_0,#tran_1_0" jquery171013312911573948127="19">best</span> <span id="tran_1_1" data-aligning="#src_1_1,#tran_1_1" jquery171013312911573948127="16">resolution</span> is 1366 X 768.</div> |
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| + | <div> ABSTRACT</div> | ||
| + | <table width="100%" border="0"> | ||
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| + | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="30%"> </td> | ||
| + | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="57%"> | ||
| + | <p> Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs still have disadvantages such as hard to determine the contaminants concentrations which is a major drawback for MFCs applications. </p> | ||
| + | <p> </p> | ||
| + | <table width="100%" border="0"> | ||
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| + | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" ><img src="https://static.igem.org/mediawiki/2014/4/46/Bigabstract1.png" width="692" height="170" /></td> | ||
| + | </tr> | ||
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| + | <p> In our study, we found that the electricity produced by MFCs was weakly correlated to the substrates' concentrations such as P-nitrophenol (PNP) in anode or Chromium (VI) in cathode. But the electricity produced was not stable and so did the correlations. Therefore, we hypothesize that a) by using genetic engineered bacteria, the MFC's electricity will be more stable and correlated with substrates concentrations. b) By monitoring MFC's electricity, it will be possible to measure substrates' concentrations.</p> | ||
| + | <p> </p> | ||
| + | <table width="100%" border="0"> | ||
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| + | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" ><img src="https://static.igem.org/mediawiki/2014/5/5f/Bigabstract2.png" alt="" width="692" height="203" /></td> | ||
| + | </tr> | ||
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| + | <p> </p> | ||
| + | <p> In this study, we've designed a novel MFC system. For anode strain, we've cloned a NO3- sensor sequence and a riboflavin producing genes into Escherichia coli. The recombinants is able to detect PNP and produce riboflavin to boost electrical generation when co-cultured with Shewanella oneidensis. In the cathode, gene codes chromate (VI) reductase Yief was cloned into E.coli. The stability of MFCs has been improved and the electricity generated was correlated with the substrates. Moreover, based on this correlation, we've designed a cell phone program which is able to remotely monitor the contaminants concentrations in MFCs. </p> | ||
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| + | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="13%"> </td> | ||
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Latest revision as of 21:45, 17 October 2014
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CONTENTS
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Background |
Wet Lab |
Dry Lab |
Parts |
Human Practice |
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Our team |
Interlab |
Safety |
Notebook |
Future Work |
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ABSTRACT
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