Team:LZU-China

From 2014.igem.org

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  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
  <div>CONTENTS</div>
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
   <table width="100%" border="0" align="center">
     <tr>
     <tr>
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       <td width="191" bgcolor="#0053A3">ABSTRACT</td>
+
       <td width="20%"><p>&nbsp;</p>
 +
      <p>&nbsp;</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/background"><img src="https://static.igem.org/mediawiki/2014/8/83/1backgroud.png" width="100" height="100" /></a></p>
 +
      <p>Background</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/wetlab"><img src="https://static.igem.org/mediawiki/2014/d/d5/2wetlab.png" width="100" height="100" /></a></p>
 +
      <p>Wet Lab</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/drylab"><img src="https://static.igem.org/mediawiki/2014/c/c6/3drylab.png" width="100" height="100" /></a></p>
 +
      <p>Dry Lab</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/Parts"><img src="https://static.igem.org/mediawiki/2014/1/14/4parts.png" width="100" height="100" /></a></p>
 +
      <p>Parts</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/hp"><img src="https://static.igem.org/mediawiki/2014/5/5f/5humanpractics.png" width="100" height="100" /></a></p>
 +
      <p>Human Practice</p></td>
 +
      <td width="20%">&nbsp;</td>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <td bgcolor="#0053A3">DATA PAGE</td>
+
       <td width="20%">&nbsp;</td>
 +
      <td width="12%"><p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p></td>
 +
      <td width="12%">&nbsp;</td>
 +
      <td width="12%">&nbsp;</td>
 +
      <td width="12%"><p>&nbsp;</p></td>
 +
      <td width="12%">&nbsp;</td>
 +
      <td width="20%">&nbsp;</td>
     </tr>
     </tr>
     <tr>
     <tr>
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       <td bgcolor="#0053A3">PARTS</td>
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       <td width="20%">&nbsp;</td>
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    </tr>
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      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/Team"><img src="https://static.igem.org/mediawiki/2014/a/a2/6members.png" width="100" height="100" /></a></p>
-
    <tr>
+
      <p>Our team</p></td>
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       <td bgcolor="#0053A3">RESULT</td>
+
       <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/interlab"><img src="https://static.igem.org/mediawiki/2014/c/cc/7interlab.png" width="100" height="100" /></a></p>
-
    </tr>
+
      <p>Interlab</p></td>
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    <tr>
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      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/Safety"><img src="https://static.igem.org/mediawiki/2014/e/ee/8safety.png" width="100" height="100" /></a></p>
-
       <td bgcolor="#0053A3">FUTURE WORK</td>
+
      <p>Safety</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/Notebook"><img src="https://static.igem.org/mediawiki/2014/0/00/9notebook.png" width="100" height="100" /></a></p>
 +
      <p>Notebook</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/future"><img src="https://static.igem.org/mediawiki/2014/0/04/10future.png" width="100" height="100" /></a></p>
 +
      <p>Future Work</p></td>
 +
       <td width="20%">&nbsp;</td>
     </tr>
     </tr>
 +
  </table>
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
  <table width="100%" border="0">
     <tr>
     <tr>
-
       <td bgcolor="#0053A3">SAFETY</td>
+
       <td><div> *In order to get better user experience, we recommend the Internet Explorer 10 (or higher version). And the <span id="tran_1_0" data-aligning="#src_1_0,#tran_1_0" jquery171013312911573948127="19">best</span> <span id="tran_1_1" data-aligning="#src_1_1,#tran_1_1" jquery171013312911573948127="16">resolution</span> is 1366 X 768.</div>
 +
        .</td>
     </tr>
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   </table>
   </table>
 +
  <p>&nbsp;</p>
</div>
</div>
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  <p>&nbsp;</p>
 +
   <table width="100%" height="627" border="0">
     <tr>
     <tr>
-
       <td width="191" bgcolor="#0053A3"><p>NOTEBOOK</p></td>
+
       <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" height="623" valign="top"><p>&nbsp;</p>
-
    </tr>
+
      <p>&nbsp;</p>
-
    <tr>
+
      <div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;ABSTRACT</div>
-
      <td bgcolor="#0053A3">INTERVIEWS </td>
+
      <table width="100%" border="0">
-
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        <tr>
-
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          <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="30%">&nbsp;</td>
-
      <td bgcolor="#0053A3">VIDEOS</td>
+
          <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="57%">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs still have disadvantages such as hard to determine the contaminants concentrations which is a major drawback for MFCs applications. </p>
 +
            <p>&nbsp;</p>
 +
            <table width="100%" border="0">
 +
              <tr>
 +
                <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" ><img src="https://static.igem.org/mediawiki/2014/4/46/Bigabstract1.png" width="692" height="170" /></td>
 +
              </tr>
 +
            </table>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In our study, we found that the electricity produced by MFCs was weakly correlated to the substrates' concentrations such as P-nitrophenol (PNP) in anode or Chromium (VI) in cathode. But the electricity produced was not stable and so did the correlations. Therefore, we hypothesize that a) by using genetic engineered bacteria, the MFC's electricity will be more stable and correlated with substrates concentrations. b) By monitoring MFC's electricity, it will be possible to measure substrates' concentrations.</p>
 +
            <p>&nbsp;</p>
 +
            <table width="100%" border="0">
 +
              <tr>
 +
                <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" ><img src="https://static.igem.org/mediawiki/2014/5/5f/Bigabstract2.png" alt="" width="692" height="203" /></td>
 +
              </tr>
 +
            </table>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In this study, we've designed a novel MFC system. For anode strain, we've cloned a NO3- sensor sequence and a riboflavin producing genes into Escherichia coli. The recombinants is able to detect PNP and produce riboflavin to boost electrical generation when co-cultured with Shewanella oneidensis. In the cathode, gene codes chromate (VI) reductase Yief was cloned into E.coli. The stability of MFCs has been improved and the electricity generated was correlated with the substrates. Moreover, based on this correlation, we've designed a cell phone program which is able to remotely monitor the contaminants concentrations in MFCs. </p>
 +
            <p><br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
 +
          </p></td>
 +
          <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="13%">&nbsp;</td>
 +
        </tr>
 +
      </table>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p></td>
     </tr>
     </tr>
   </table>
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+
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      <td height="12" bgcolor="#0053A3"><a href="https://2013.igem.org/Team:LZU-China">iGEM LZU-CHINA 2013</a></td>
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<span class="icon"></span>
 +
<span class="text">Dry Lab</span>
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<a class="li note" href="https://2014.igem.org/Team:LZU-China/Parts">
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<span class="icon"></span>
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<span class="text">Parts</span>
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                    <a class="li duanxin" href="https://2014.igem.org/Team:LZU-China/interlab">
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<span class="text">Interlab</span>
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<a class="li zhaohui" href="https://2014.igem.org/Team:LZU-China/future">
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<a class="def-nav" href="https://2014.igem.org/Team:LZU-China/Safety">Safety</a>
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<a class="def-nav" href="https://2014.igem.org/Team:LZU-China/hp">H. Practice</a>
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<a class="f-icon identity" href="https://2014.igem.org/Team:LZU-China/Team" title="Our team"></a>
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<span class="name top-username">Our Team</span>
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<span class="li"><a href="https://2014.igem.org/Team:LZU-China/Team">Team Members</a></span>
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<span class="li"><a href="https://2014.igem.org/Team:LZU-China/Instructors">Advisors</a></span>
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<span class="li"><a href="https://2014.igem.org/Team:LZU-China/Instructors">Instructors</a></span>
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<span class="li"><a href="https://2014.igem.org/Team:LZU-China/Attribution">Attribution</a></span>
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<span class="li"><a href="https://2014.igem.org/Team:LZU-China/acknowledgement">Acknowledgements</a></span>
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<span class="li"><a href="https://igem.org/Team.cgi?year=2014&team_name=LZU-China">Team Profile</a></span>
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<span class="li"><a href="http://en.lzu.edu.cn/">Lanzhou University</a></span>
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<span class="li"><a href="https://2013.igem.org/Team:LZU-China">LZU-CHINA 2013</a></span>
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  <p><strong>欢迎访问国际遗传学工程机器大赛(<SPAN lang="EN-US">iGEM</SPAN>)兰州大学团队主页!<SPAN lang="EN-US"><BR>
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    Welcome to visit the homepage of iGEM LZU-China!</SPAN><SPAN lang="EN-US"></SPAN></strong><br>
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  <SPAN id="tran_5_0" jQuery17105175891956959193="41" data-aligning="#src_5_0,#tran_5_0"><img src="https://static.igem.org/mediawiki/2014/c/cc/Logolzuchina.jpg" width="450" height="450"><br>
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  相关页面正在建设中,更多精彩内容敬请期待!<br>
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  This</SPAN> <SPAN id="tran_5_1" data-aligning="#src_5_1,#tran_5_1">wiki</SPAN> <SPAN id="tran_5_2" jQuery17105175891956959193="39" data-aligning="#src_5_2,#tran_5_2">is  under</SPAN> <SPAN id="tran_5_3" jQuery17105175891956959193="40" data-aligning="#src_5_3,#tran_5_3">construction</SPAN>. <SPAN id="tran_1_2" data-aligning="#src_1_2,#tran_1_2">More</SPAN> <SPAN id="tran_1_3" data-aligning="#src_1_3,#tran_1_3">pages</SPAN> will be updated!  </p>
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  <p>LanZhou University | 222 TianShui South Road, LanZhou, China, 730000<br>
 
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<h1 >WELCOME TO LZU-iGEM 2014! </h1>
 
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<p>How many roads must a man walk down
 
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world!
 
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:LZU-China&action=edit"style="color:#FFFFFE"> Click here  to edit this page!</a> </p>
 
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<a href="https://2014.igem.org/Team:LZU-China"style="color:#000000">Home </a> </td>
 
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<a href="https://2014.igem.org/Team:LZU-China/Notebook"style="color:#000000"> Notebook</a></td>
 
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<ul>
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<li><a href="https://2014.igem.org/Team:LZU-China">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:LZU-China/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=LZU-China">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:LZU-China/Project">Project</a> </li>
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<li><a href="https://2014.igem.org/Team:LZU-China/Parts">Parts</a> </li>
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<li><a href="https://2014.igem.org/Team:LZU-China/Modeling">Modeling</a> </li>
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<li><a href="https://2014.igem.org/Team:LZU-China/Notebook">Notebook</a> </li>
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<li>As part of your documentation, keep the links from the menu to the left. </li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<p>We are currently working on providing teams with some easy to use design templates.
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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<li>Start documenting your project as early as possible; don't leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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Latest revision as of 21:45, 17 October 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> LZU-China 2014

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

CONTENTS

 

 

 

 

 

Background

Wet Lab

Dry Lab

Parts

Human Practice

 
 

 

 

 

   

 

   
 

Our team

Interlab

Safety

Notebook

Future Work

 

 

 

*In order to get better user experience, we recommend the Internet Explorer 10 (or higher version). And the best resolution is 1366 X 768.
.

 

 

 

 

        ABSTRACT
              

            Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs still have disadvantages such as hard to determine the contaminants concentrations which is a major drawback for MFCs applications.

 

 

            In our study, we found that the electricity produced by MFCs was weakly correlated to the substrates' concentrations such as P-nitrophenol (PNP) in anode or Chromium (VI) in cathode. But the electricity produced was not stable and so did the correlations. Therefore, we hypothesize that a) by using genetic engineered bacteria, the MFC's electricity will be more stable and correlated with substrates concentrations. b) By monitoring MFC's electricity, it will be possible to measure substrates' concentrations.

 

 

            In this study, we've designed a novel MFC system. For anode strain, we've cloned a NO3- sensor sequence and a riboflavin producing genes into Escherichia coli. The recombinants is able to detect PNP and produce riboflavin to boost electrical generation when co-cultured with Shewanella oneidensis. In the cathode, gene codes chromate (VI) reductase Yief was cloned into E.coli. The stability of MFCs has been improved and the electricity generated was correlated with the substrates. Moreover, based on this correlation, we've designed a cell phone program which is able to remotely monitor the contaminants concentrations in MFCs.