Team:Heidelberg/Project/Achievements

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            <p style="font-weight:light; font-size:30px">Providing inteins as NEW TOOL for the scientific community.</p>
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              <p style="margin-left:50px; font-size:20px">Creating a toolbox including all intein functions!</p>
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  <h1>Bronze</h1>
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    <li>Please find a comprehensive compilation of <a href="http://2014.igem.org/Team:Heidelberg/Team/Sponsoring">sponsors</a>, partners and scientific contributors on our <a href="http://2014.igem.org/Team:Heidelberg/Team/Attributions">acknowledgements page</a>. </li>
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    <li>We also encourage you to take notice of the projects “Photo-intein” and “Mito-intein” by <a href="http://2014.igem.org/Team:Queens_Canada/Project">iGEM team Queens from Canada</a> that may supply you with complementary information and tools for the use of inteins in synthetic biology!</li>
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    <li>A list of links to more than 60 parts in the registry submitted by our team (being or not being part of the new intein toolbox) can be found <a href="http://2014.igem.org/Team:Heidelberg/Parts#allParts">here</a>.</li>
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  <h1>Silver</h1>
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    <li>We experimentally validated that our biobricks <a href="http://parts.igem.org/Part:BBa_K1362000">BBa_K1362000</a>, <a href="http://parts.igem.org/Part:BBa_K1362100">BBa_K1362100 </a>and <a href="http://parts.igem.org/Part:BBa_K1362101">BBa_K1362101</a> work as expected. For more information on the <a href="http://2014.igem.org/Team:Heidelberg/Parts#Favorite Parts">parts</a> please visit the corresponding main pages in the parts registry or explore their involvement in our subprojects.</li>
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    <li>Religious perceptions of synthetic biology have been part of several surveys during the past ten years of iGEM and Human Practices projects. Since religious groups cover the majority of worlds population, deliver moral values and wield power at the same time, we decided to dedicate a whole event on the topic of religion, philosophy and ethics regarding synthetic biology. Please find an <a href="http://2014.igem.org/Team:Heidelberg/Human_Practice/Ethics">evaluation of our event</a> on the corresponding Human Practices pages. <!--In order to reassure ourselves about the acceptability of our project and synthetic biology in general, we also used this opportunity to build up on the work of the iGEM Team Heidelberg 2013 and conducted a survey that addresses basic questions regarding the public reflection of our work.-->
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  <h1>Gold</h1>
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  <li>We improved the function of the <b>already existing</b> biobrick part <a href="http://parts.igem.org/Part:BBa_K117505">BBa_K1175005 </a>by optimizing and resubmitting the corresponding sequence of B. subtilis xylanase to the registry (Part:<a href="http://parts.igem.org/Part:BBa_K1362020"> BBa_K1362020</a>). In addition, we submitted a new part for expression of <a href="http://parts.igem.org/Part:BBa_K1362022">circularized xylanase </a>(BBa_K1362022) that might be used in future applications with need for refined enzyme stability.</li>
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  <li>Despite the fact that we focused on building a set of powerful soft- and wetware tools to help future iGEM-teams developing and realizing projects in synthetic biology, we are happy to announce that we were also able to help out several team during the course of our project, aspecially with sending <a href="http://2014.igem.org/Team:Heidelberg/Parts#Backbones"> our expression vectors</a>. Read more abou in in our <a href="http://2014.igem.org/Team:Heidelberg/Team/Collaborations">Collaborations</a>.</li>
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  <li>Concepts developed and information gathered during the last decade by iGEM teams from all over the world proof efforts in Human Practices worth to be rewarded with a track of its own in this years iGEM competition. Since iGEM teams from Heidelberg have always been interested in the question how laymen citizens can be involved into synthetic biology, the Heidelberg teams already introduced new concepts to the community to improve communication (first abstract page in multiple languages, first site tour for laymen, first mascot 2008), do research on the mechanisms of science communication (2010) and encourage reflection by philosophers and artists as representers of society (2010, 2013). In the style of of the new iGEM community labs track that involves science amateurs “beyond the accolades of scientific publishing and economic reward”, we sought for a new way to involve laymen in actual science and build a strong community of well informed supporters and communicators of synthetic biology at the same time. Now we proudly present the crowd sourcing and communication platform igem@home.</li>
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Revision as of 11:06, 17 October 2014

– Creating the new intein standard for the iGEM community.

Establishing protein circularization as a NEW BIOENGINEERING TOOL in synthetic biology.

Contributing to iGEM with a new foundational advance!

Providing a NEW COMPREHENSIVE TOOLBOX based on inteins for modifying proteins post-translationally.

Sending 67 Biobricks to Registry of Biological parts!

Development of a NEW STANDARD to make the use of inteins easy and modular.

Establishment of a new RFC!

Showing that the toolbox WORKS: proteins are circularized and split fluorescent proteins are reconstituted.

Making Gels, Western Blots, Fluorescence-based Assays and Mass spectrometry to prove it!

Creating circular DNMT1 and showing that it is ACTIVE.

For the first time achieving the circularization of a large protein!

Developing a NEW SOFTWARE to calculate customized linkers to circularize proteins.

Making CRAUT open-source for the scientific community.

Establish a distributed computing platform called iGEM@home.

Using this platform as an entirely new way to reach out to the world with synthetic biology concepts!

Creating a NEW SOFTWARE to display the notebook on the wiki.

Distributing MidNightDOC to the iGEM community to help future teams organize their protocols!

Bronze


  • Please find a comprehensive compilation of sponsors, partners and scientific contributors on our acknowledgements page.

  • We also encourage you to take notice of the projects “Photo-intein” and “Mito-intein” by iGEM team Queens from Canada that may supply you with complementary information and tools for the use of inteins in synthetic biology!

  • A list of links to more than 60 parts in the registry submitted by our team (being or not being part of the new intein toolbox) can be found here.

Silver


  • We experimentally validated that our biobricks BBa_K1362000, BBa_K1362100 and BBa_K1362101 work as expected. For more information on the parts please visit the corresponding main pages in the parts registry or explore their involvement in our subprojects.

  • Religious perceptions of synthetic biology have been part of several surveys during the past ten years of iGEM and Human Practices projects. Since religious groups cover the majority of worlds population, deliver moral values and wield power at the same time, we decided to dedicate a whole event on the topic of religion, philosophy and ethics regarding synthetic biology. Please find an evaluation of our event on the corresponding Human Practices pages.

Gold

  • We improved the function of the already existing biobrick part BBa_K1175005 by optimizing and resubmitting the corresponding sequence of B. subtilis xylanase to the registry (Part: BBa_K1362020). In addition, we submitted a new part for expression of circularized xylanase (BBa_K1362022) that might be used in future applications with need for refined enzyme stability.

  • Despite the fact that we focused on building a set of powerful soft- and wetware tools to help future iGEM-teams developing and realizing projects in synthetic biology, we are happy to announce that we were also able to help out several team during the course of our project, aspecially with sending our expression vectors. Read more abou in in our Collaborations.

  • In the style of of the new iGEM community labs track that involves science amateurs “beyond the accolades of scientific publishing and economic reward”, we sought for a new way to involve laymen in actual science and build a strong community of well informed supporters and communicators of synthetic biology at the same time. Now we proudly present the crowd sourcing and communication platform iGEM@home.