"
Team:Colombia/How to parts
From 2014.igem.org
How to parts
Primers used to construct XXXXXXX
Name
Sequence
FW pqrr4
CTT GTC TAG ATT GCA TTT TGC AAA TTT TG
RV pqrr4
GGT CAC TAG TCA ATC TAA TAA ATG CAG ATT
FW LuxOD47E
CAA TTC TAG AAA AGA GGA GAA ATA CTA GAT G
RV LuxOD47E
ATA TAC TAG TTG TGA CTC TAG TAG AGA
BioBricks used to construct XXXXXXX
Name
Description
Developer team
BBa_K581010
Regulatory proteins for receiving signaling of CAI-1
Peking_Signaling 2011
BBa_K581011
A generator of CAI-1 behind pBAD promoter
Peking_Signaling 2011
BBa_K581009
GFP behind promoter qrr
Peking_Signaling 2011
BBa_K218017
LuxO D47E under constitutive expression of TetR repressible promoter
Calgary 2009
BBa_K592025
Purple-blue chromoprotein amilCP
Uppsala-Sweden 2011
BBa_K518012
RFP
UT-Tokyo 2011
BBa_K206000
pBAD strong(arabinose inducible promoter)
UT-Tokyo British_Columbia 2009
BBa_Q04400
TetR QPI (inverter)
Antiquity 2003
How to parts
| Name | Sequence |
| FW pqrr4 | CTT GTC TAG ATT GCA TTT TGC AAA TTT TG |
| RV pqrr4 | GGT CAC TAG TCA ATC TAA TAA ATG CAG ATT |
| FW LuxOD47E | CAA TTC TAG AAA AGA GGA GAA ATA CTA GAT G |
| RV LuxOD47E | ATA TAC TAG TTG TGA CTC TAG TAG AGA |
| Name | Description | Developer team |
| BBa_K581010 | Regulatory proteins for receiving signaling of CAI-1 | Peking_Signaling 2011 |
| BBa_K581011 | A generator of CAI-1 behind pBAD promoter | Peking_Signaling 2011 |
| BBa_K581009 | GFP behind promoter qrr | Peking_Signaling 2011 |
| BBa_K218017 | LuxO D47E under constitutive expression of TetR repressible promoter | Calgary 2009 |
| BBa_K592025 | Purple-blue chromoprotein amilCP | Uppsala-Sweden 2011 |
| BBa_K518012 | RFP | UT-Tokyo 2011 |
| BBa_K206000 | pBAD strong(arabinose inducible promoter) | UT-Tokyo British_Columbia 2009 |
| BBa_Q04400 | TetR QPI (inverter) | Antiquity 2003 |
