Team:Tokyo Tech/Safety

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<title>Tokyo_Tech</title>
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<a href="https://2014.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/thumb/8/84/Tokyo_Tech_iGEM_logo.png/794px-Tokyo_Tech_iGEM_logo.png" width="90" /></a></div>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech">Home</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Project">Project</a></li>
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<li><a href="#">Experiment</a>
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  <ul>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Plux and Prhl reporter assay" style="width:400px; margin-left:-135px;">Plux and Prhl reporter assay</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay" style="width:400px; margin-left:-135px;">Improved Prhl reporter assay</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/C4HSL-dependent_3OC12HSL_production" style="width:400px; margin-left:-135px;">C4HSL-dependent 3OC12HSL production</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/3OC12HSL-dependent_C4HSL_production" style="width:400px; margin-left:-135px;">3OC12HSL-dependent C4HSL production</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Symbiosis_confirmation_by_co-culture" style="width:400px; margin-left:-135px;">Mutualism Confirmation ~Co-culture Assay~</a></li>
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  </ul>
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<li><a href="#">Modeling</a>
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                          <ul>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Overview"  style="width:400px; margin-left:-135px;">Overview</a></li>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Growth Conditions For Company And Customer"  style="width:400px; margin-left:-135px;">Growth Conditions For Company And Customer</a></li>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Analysis of C4HSL-dependent Switch" style="width:400px; margin-left:-135px;">Analysis of C4HSL-dependent Switch</a></li>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Economic Wave"  style="width:400px; margin-left:-135px;">Economic Wave</a></li>
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                          </ul>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">Parts</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices" style="height:50px; padding-top:3px;">Policy and Practices</a></li>
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<li class="current_page_item"><a href="#">More</a>
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    <ul>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/About_Us" style="width:240px; margin-left:-53px;">About Us</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Safety" style="width:240px; margin-left:-53px;">Safety</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Attribution_and_Contribution" style="width:240px; margin-left:-53px;">Attribution and Contribution</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Collaboration" style="width:240px; margin-left:-53px;">Collaboration</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Judging_Form" style="width:240px; margin-left:-53px;">Judging Form</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Sponsors" style="width:240px; margin-left:-53px;">Sponsors</a></li>
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        <li><a href="https://igem.org/Team.cgi?id=1529" style="width:240px; margin-left:-53px;">Official Team Page</a></li>
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      <h2 class="title">Safety</h2>
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            <span class="meta">Safety information for our organisms </span>
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              <td>&nbsp;</td>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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              <td class="title-small"><div align="center">Safety Form </div></td>
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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              <td>&nbsp;</td>
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              <td class="head">1.) Your Training </td>
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              <td class="info-18"><strong>a) Have your team members received any safety training yet?</strong></td>
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              <td class="info">- Yes,we have already received safety training.</td>
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<a href="https://2014.igem.org/Team:Tokyo_Tech"style="color:#000000">Home </a> </td>
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              <td>&nbsp;</td>
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<a href="https://2014.igem.org/Team:Tokyo_Tech/Team"style="color:#000000"> Team </a> </td>
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              <td class="info-18"><strong>b) Please briefly describe the topics that you learned about (or will learn about) in your safety training.</strong></td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Tokyo_Tech"style="color:#000000"> Official Team Profile </a></td>
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              <td class="info">- Microbiological experiments (including recombinant DNA) Safety in Recombinant DNA Experiments </td>
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<a href="https://2014.igem.org/Team:Tokyo_Tech/Project"style="color:#000000"> Project</a></td>
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              <td>&nbsp;</td>
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<a href="https://2014.igem.org/Team:Tokyo_Tech/Parts"style="color:#000000"> Parts</a></td>
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              <td class="info-18"><strong>c) Please give a link to the laboratory safety  training requirements of your institution (college, university,  community lab, etc). Or, if you cannot give a link, briefly describe the  requirements.</strong></td>
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<a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling"style="color:#000000"> Modeling</a></td>
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              <td class="info">- SAFETY HANDBOOK wrote by Committee for Practice on management of Safety in Tokyo Institure Microbiological experiments (including recombinant DNA) </td>
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<a href="https://2014.igem.org/Team:Tokyo_Tech/Notebook"style="color:#000000"> Notebook</a></td>
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              <td>&nbsp;</td>
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<a href="https://2014.igem.org/Team:Tokyo_Tech/Safety"style=" color:#000000"> Safety </a></td>
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              <td>&nbsp;</td>
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<a href="https://2014.igem.org/Team:Tokyo_Tech/Attributions"style="color:#000000"> Attributions </a></td>
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              <td class="head">2.) Your Local Rules and Regulations</td>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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              <td class="info-18"><strong>a) Who is responsible for biological safety at  your institution? (You might have an Institutional Biosafety Committee,  an Office of Environmental Health and Safety, a single Biosafety  Officer, or some other arrangement.) Have you discussed your project  with them? Describe any concerns they raised, and any changes you made  in your project based on your discussion.</strong></td>
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              <td class="info">- Our institute has recombinant DNA committee </td>
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              <td>&nbsp;</td>
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              <td class="info-18"><strong>b) What are the biosafety guidelines of your  institution? Please give a link to these guidelines, or briefly describe  them if you cannot give a link.</strong></td>
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<tr><td > <h3> Welcome! </h3></td>
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<td ></td >
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<td > <h3> Timeline</h3></td>
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              <td class="info">- <a href="http://www.rpd.titech.ac.jp//rpdiv/somu/docs/idenshikumikaejikken_anzenkanrikisoku.pdf">http://www.rpd.titech.ac.jp//rpdiv/somu/docs/idenshikumikaejikken_anzenkanrikisoku.pdf</a> </td>
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              <td>&nbsp;</td>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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              <td class="info-18"><strong>c) In your country, what are the regulations  that govern biosafety in research laboratories? Please give a link to  these regulations, or briefly describe them if you cannot give a link.</strong></td>
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              <td class="info"><a href="http://www.lifescience.mext.go.jp/bioethics/anzen.html">- http://www.lifescience.mext.go.jp/bioethics/anzen.html</a></td>
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<h3> Edit this page!</h3>
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
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              <td>&nbsp;</td>
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<li> <a href=" ">Learn about lab Safety for Today</a></li>  
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<li> <a href="">Learn about Safety for the future of your project.</a></li>  
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              <td><span class="head">3.) The Organism and Parts that You Use </span></td>
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<h3> Your Lab </h3>
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              <td class="entry">&nbsp;</td>
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>  
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Image:Example2_Lab_1.png|The building our lab is in!
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Image:Example2_Lab_2.png|The inside of our lab!
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Image:Example2_Lab_3.png|Team Member 3 doing an experiment
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Image:Example2_Lab_4.png|Working in biosafety cabinets
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              <td><h1>*Species: <i>E. coli</i> MG1655 </h1></td>
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Image:Example2_Lab_5.png|Team all gloved up and ready for work!
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Image:Example2_Lab_6.png|Equipment that we use to do SCIENCE!
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Image:Example2_Lab_7.png|We decorated this part of our lab
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Image:Example2_Lab_8.png|Whatever else you want
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              <td><h3 class="info-18">*Risk Group: 1</h3>
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                <h3 class="info-18">*Risk Group Source: <a href="http://osp.od.nih.gov/office-biotechnology-activities/biosafety/nih-guidelines">NIH Guidelines </a></h3>
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                <h3 class="info-18"> *Disease risk to humans:No</h3>
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                <h3 class="info-18">*Part number/name: ppX</h3>
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                <h3 class="info-18">*Natural function of part: exopolyphosphatase</h3>
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                <h3 class="info-18">*How did you acquire it: made by PCR</h3>
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                <h3 class="info-18">*How will you use it?:  exopolyphosphatase</h3></td>
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<li> <b>Now :</b> Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
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              <td>&nbsp;</td>
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
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              <td><h1>*Species: <i>E. coli</i> MG1655 </h1></td>
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
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              <td>&nbsp;</td>
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
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<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
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              <td><h3 class="info-18">*Risk Group: 1</h3>
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                  <h3 class="info-18">*Risk Group Source: <a href="http://osp.od.nih.gov/office-biotechnology-activities/biosafety/nih-guidelines">NIH Guidelines </a> </h3>
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                  <h3 class="info-18"> *Disease risk to humans:No</h3>
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                  <h3 class="info-18">*Part number/name: phoU </h3>
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                  <h3 class="info-18">*Natural function of part: regulation of the phosphate regulon</h3>
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                  <h3 class="info-18">*How did you acquire it: made by PCR</h3>
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                  <h3 class="info-18">*How will you use it?:  regulation of the phosphate regulon</h3></td>
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              <td><h1>*Species: <i>E. coli</i> MG1655 </h1></td>
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              <td><h3 class="info-18">*Risk Group: 1</h3>
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</table>
+
                  <h3 class="info-18">*Risk Group Source: <a href="http://osp.od.nih.gov/office-biotechnology-activities/biosafety/nih-guidelines">NIH Guidelines </a> </h3>
 +
                <h3 class="info-18"> *Disease risk to humans:No</h3>
 +
                <h3 class="info-18">*Part number/name: ydeD </h3>
 +
                <h3 class="info-18">*Natural function of part:  transportation of cystein</h3>
 +
                <h3 class="info-18">*How did you acquire it: made by PCR</h3>
 +
                <h3 class="info-18">*How will you use it?: transportation of cystein</h3></td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td><h1>*Species: <i>E. coli</i> MG1655 </h1></td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td><h3 class="info-18">*Risk Group: 1</h3>
 +
                  <h3 class="info-18">*Risk Group Source: <a href="http://osp.od.nih.gov/office-biotechnology-activities/biosafety/nih-guidelines">NIH Guidelines </a> </h3>
 +
                <h3 class="info-18"> *Disease risk to humans:No</h3>
 +
                <h3 class="info-18">*Part number/name: yfiK</h3>
 +
                <h3 class="info-18">*Natural function of part: transportation of cystein</h3>
 +
                <h3 class="info-18">*How did you acquire it: made by PCR</h3>
 +
                <h3 class="info-18">*How will you use it?:  transportation of cystein</h3></td>
 +
            </tr>
 +
           
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td><h1>*Species: <i>E. coli</i> MG1655 </h1></td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td><h3 class="info-18">*Risk Group: 1</h3>
 +
                  <h3 class="info-18">*Risk Group Source: <a href="http://osp.od.nih.gov/office-biotechnology-activities/biosafety/nih-guidelines">NIH Guidelines </a> </h3>
 +
                <h3 class="info-18"> *Disease risk to humans:No</h3>
 +
                <h3 class="info-18">*Part number/name: rpoS </h3>
 +
                <h3 class="info-18">*Natural function of part:  regulation of transcription</h3>
 +
                <h3 class="info-18">*How did you acquire it: made by PCR</h3>
 +
                <h3 class="info-18">*How will you use it?: regulation of transcription, growth inhibition</h3></td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td><h1>*Species: <i>E. coli</i> MG1655 </h1></td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td><h3 class="info-18">*Risk Group: 1</h3>
 +
                  <h3 class="info-18">*Risk Group Source: <a href="http://osp.od.nih.gov/office-biotechnology-activities/biosafety/nih-guidelines">NIH Guidelines </a> </h3>
 +
                <h3 class="info-18"> *Disease risk to humans:No</h3>
 +
                <h3 class="info-18">*Part number/name: crl</h3>
 +
                <h3 class="info-18">*Natural function of part:  regulation of transcription, activagtor of rpoS</h3>
 +
                <h3 class="info-18">*How did you acquire it: made by PCR</h3>
 +
                <h3 class="info-18">*How will you use it?: regulation of transcription, activagtor of rpoS</h3></td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td><h1>*Species: Burkholderia glumae </h1></td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td><h3 class="info-18">*Risk Group: 2 </h3>
 +
                  <h3 class="info-18">*Risk Group Source: <a href="http://osp.od.nih.gov/office-biotechnology-activities/biosafety/nih-guidelines">NIH Guidelines </a> </h3>
 +
                  <h3 class="info-18"> *Disease risk to humans:No</h3>
 +
                  <h3 class="info-18">*Part number/name: obcA </h3>
 +
                  <h3 class="info-18">*Natural function of part:  synthesis of oxalic acid</h3>
 +
                  <h3 class="info-18">*How did you acquire it: Gene synthesis by IDT</h3>
 +
                <h3 class="info-18">*How will you use it?:  synthesis of oxalic acid</h3></td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td><h1>*Species: <i>E. coli</i> MG1655 </h1></td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td><h3 class="info-18">*Risk Group: 2 </h3>
 +
                  <h3 class="info-18">*Risk Group Source: <a href="http://osp.od.nih.gov/office-biotechnology-activities/biosafety/nih-guidelines">NIH Guidelines </a> </h3>
 +
                  <h3 class="info-18"> *Disease risk to humans:No</h3>
 +
                  <h3 class="info-18">*Part number/name: obcB</h3>
 +
                  <h3 class="info-18">*Natural function of part:  synthesis of oxalic acid</h3>
 +
                  <h3 class="info-18">*How did you acquire it: Gene synthesis by IDT</h3>
 +
                <h3 class="info-18">*How will you use it?:  synthesis of oxalic acid</h3></td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="head">4.) Risks of Your Project Now </td>
 +
            </tr>
 +
            <tr>
 +
              <td class="entry">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info-18"><strong>a) Risks to the safety and health of team members, or other people working in the lab:</strong></td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info">- Without drinking large amount of sample, there is no risk.Such taking might cause irritation to skin,eyes,and respiratory tract.</td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info-18"><strong>b) Risks to the safety and health of the general public (if any biological materials escaped from your lab):</strong></td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info">- Wihtout drinking large amount of sample, there is no risk.Such taking might cause irritation to skin,eyes,and respiratory tract.</td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info-18"><strong>c) Risks to the environment (from waste disposal, or from materials escaping from your lab):</strong></td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info">- Production of AHL might perturbate communications in wild bacteria.</td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info-18"><strong>d) Risks to security through malicious mis-use by individuals, groups, or countries:</strong></td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info">- Use of plant hormone in the opposite direction could disrupt growth of crops.Malicious usage of phage might pertubate intestinal flora.</td>
 +
            </tr>
 +
            <tr>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info-18"><strong>e) What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)</strong></td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info">- Do not eat,drink,smoke,prepare or store foods in laboratories.<br />
 +
                     -
 +
                Wear a lab-coat(white coat) while you are in a bio-lab.<br />
 +
                     -                Keep the lab tidy and clean.<br />
 +
                     -                Put labels on containers.<br />
 +
                   -              Practice disinfection,sterilization,and washing hands.</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="head">5.) Risks of Your Project in the Future </td>
 +
            </tr>
 +
            <tr>
 +
              <td class="entry">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info-18"><strong>a) What <em>new</em> risks might arise from your  project's growth? (Consider the categories of risk listed in parts a-d  of the previous question: lab workers, the general public, the  environment, and malicious mis-uses.) Also, what risks might arise if  the <em>knowledge</em> you generate or the <em>methods</em> you develop became widely available?</strong></td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info">- Excess produce of oxalic acid may cause urinary stone.(a)<br />
 +
                     -
 +
                We can farm any poisonous plants easily. (b)<br />
 +
                     -
 +
                Too many phosphate may cause contamination of environment.(c)<br />
 +
                   -
 +
              Diabetes patient may have danger if he or she intakes Cystein over 1500mg.(a)</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info-18"><strong>b) Does your project currently include any  design features to reduce risks? Or, if you did all the future work to  make your project grow into a popular product, would you plan to design  any new features to minimize risks? (For example: auxotrophic chassis,  physical containment, etc.) Such features are not required for an iGEM  project, but many teams choose to explore them.</strong></td>
 +
            </tr>
 +
            <tr>
 +
              <td class="info">- We have to consider potential risks in Bioremediation.
 +
              When our project grow, limitation of solid use will be needed.</td>
 +
            </tr>
 +
          </table>
 +
          </div>
 +
    </div>
 +
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 +
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Latest revision as of 01:22, 18 October 2014

Tokyo_Tech

Safety

Safety information for our organisms
 
Safety Form
 
 
1.) Your Training
 
a) Have your team members received any safety training yet?
- Yes,we have already received safety training.
 
b) Please briefly describe the topics that you learned about (or will learn about) in your safety training.
- Microbiological experiments (including recombinant DNA) Safety in Recombinant DNA Experiments
 
c) Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.
- SAFETY HANDBOOK wrote by Committee for Practice on management of Safety in Tokyo Institure Microbiological experiments (including recombinant DNA)
 
 
2.) Your Local Rules and Regulations
 
a) Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety, a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.
- Our institute has recombinant DNA committee
 
b) What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.
- http://www.rpd.titech.ac.jp//rpdiv/somu/docs/idenshikumikaejikken_anzenkanrikisoku.pdf
 
c) In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.
- http://www.lifescience.mext.go.jp/bioethics/anzen.html
 
 
3.) The Organism and Parts that You Use
 
 

*Species: E. coli MG1655

 

*Risk Group: 1

*Risk Group Source: NIH Guidelines

*Disease risk to humans:No

*Part number/name: ppX

*Natural function of part: exopolyphosphatase

*How did you acquire it: made by PCR

*How will you use it?: exopolyphosphatase
 

*Species: E. coli MG1655

 

*Risk Group: 1

*Risk Group Source: NIH Guidelines

*Disease risk to humans:No

*Part number/name: phoU

*Natural function of part: regulation of the phosphate regulon

*How did you acquire it: made by PCR

*How will you use it?: regulation of the phosphate regulon
 

*Species: E. coli MG1655

 

*Risk Group: 1

*Risk Group Source: NIH Guidelines

*Disease risk to humans:No

*Part number/name: ydeD

*Natural function of part: transportation of cystein

*How did you acquire it: made by PCR

*How will you use it?: transportation of cystein
 

*Species: E. coli MG1655

 

*Risk Group: 1

*Risk Group Source: NIH Guidelines

*Disease risk to humans:No

*Part number/name: yfiK

*Natural function of part: transportation of cystein

*How did you acquire it: made by PCR

*How will you use it?: transportation of cystein
 

*Species: E. coli MG1655

 

*Risk Group: 1

*Risk Group Source: NIH Guidelines

*Disease risk to humans:No

*Part number/name: rpoS

*Natural function of part: regulation of transcription

*How did you acquire it: made by PCR

*How will you use it?: regulation of transcription, growth inhibition
 

*Species: E. coli MG1655

 

*Risk Group: 1

*Risk Group Source: NIH Guidelines

*Disease risk to humans:No

*Part number/name: crl

*Natural function of part: regulation of transcription, activagtor of rpoS

*How did you acquire it: made by PCR

*How will you use it?: regulation of transcription, activagtor of rpoS
 

*Species: Burkholderia glumae

 

*Risk Group: 2

*Risk Group Source: NIH Guidelines

*Disease risk to humans:No

*Part number/name: obcA

*Natural function of part: synthesis of oxalic acid

*How did you acquire it: Gene synthesis by IDT

*How will you use it?: synthesis of oxalic acid
 

*Species: E. coli MG1655

 

*Risk Group: 2

*Risk Group Source: NIH Guidelines

*Disease risk to humans:No

*Part number/name: obcB

*Natural function of part: synthesis of oxalic acid

*How did you acquire it: Gene synthesis by IDT

*How will you use it?: synthesis of oxalic acid
 
 
4.) Risks of Your Project Now
 
a) Risks to the safety and health of team members, or other people working in the lab:
- Without drinking large amount of sample, there is no risk.Such taking might cause irritation to skin,eyes,and respiratory tract.
 
b) Risks to the safety and health of the general public (if any biological materials escaped from your lab):
- Wihtout drinking large amount of sample, there is no risk.Such taking might cause irritation to skin,eyes,and respiratory tract.
 
c) Risks to the environment (from waste disposal, or from materials escaping from your lab):
- Production of AHL might perturbate communications in wild bacteria.
 
d) Risks to security through malicious mis-use by individuals, groups, or countries:
- Use of plant hormone in the opposite direction could disrupt growth of crops.Malicious usage of phage might pertubate intestinal flora.
 
e) What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)
- Do not eat,drink,smoke,prepare or store foods in laboratories.
     - Wear a lab-coat(white coat) while you are in a bio-lab.
     - Keep the lab tidy and clean.
     - Put labels on containers.
     - Practice disinfection,sterilization,and washing hands.
 
5.) Risks of Your Project in the Future
 
a) What new risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?
- Excess produce of oxalic acid may cause urinary stone.(a)
     - We can farm any poisonous plants easily. (b)
     - Too many phosphate may cause contamination of environment.(c)
     - Diabetes patient may have danger if he or she intakes Cystein over 1500mg.(a)
 
b) Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.) Such features are not required for an iGEM project, but many teams choose to explore them.
- We have to consider potential risks in Bioremediation. When our project grow, limitation of solid use will be needed.
 

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