Team:Tokyo Tech/Attribution and Contribution

From 2014.igem.org

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Latest revision as of 01:22, 18 October 2014

Tokyo_Tech

Attribution and Contribution

We could not reach this point without anyone


Attribution

We are proud to say that all the ideas we worked on this year were proposed by undergraduate members of our team Tokyo_Tech 2014. Moreover, the whole experimental data created by our team comes from experiments all carried out by undergraduates. The project "Bank E. coli" was proposed by Naoto Takahashi. Modeling related to this project was done by Takuya Kato and Ye Kai. We want to thank every advisers and instructors who helped us with discussing these ideas and guided us in the experiments.

Student team members

All cloning (transformation, culture cells, mini prep):
- Yuta Kino, Yuya Nakamura, Kohdai Hibi with helping of other team members

Parts construction (restriction digest, ligation):
- Yuta Kino, Yuya Nakamura, Kohdai Hibi

AHL-dependent gene expression assay:
- Shoko Suzuki, Naoto Takahashi, Keina Matsumura

Co-culture assay:
- Yuya Nakamura, Gyomon Ryu

Improved rhl promoter assay:
- Naoto Takahashi, Keina Matsumura, Ayaka Murase

Modeling (simulation of the genetic circuit):
- Takuya Kato, Kai Ye

Wiki and slide design:
- Paniti Achararit, Kai Ye, Riku Shinohara

Policy and Practices:
- Miyabi Hishinuma, Ayaka Murase with helping of other team members

Contribution

General Support

Prof. Kiga let us use his lab for all of our experiments. He also has led many Tokyo Tech teams to the excellent results in iGEM since this competition has started.
Prof. Yamamura and Prof. Konagaya supported us in many ways to give us a better researching environment.
Prof. Ayukawa supported us in all of our experiments throughout the preparations for iGEM.
Promega KK provided us a part of the restriction enzyme we used without any charge.

Instrumental Analysis Support

Biomaterial Analysis Center, Technical Department, checked the sequence of the plasmids which we had asked.

Policy and Practice Support

Tomoya Saito, who is working for National Institute of Public Health, gave us a lecture on dual-use technologies, and gave us useful advice about biosafety.
Hiromichi Ando arranged MUSE TALK and let us present there.
Akihiro Hiyoshi, who is the principal of Ohyamaguchi Elementary School, gave us a chance to communicate with students.

Presentation Adviser

We would like to thank all the people who came to check our presentations and gave us useful feedbacks:
Professor: Masaaki Wachi, Hiroshi Iwasaki, Masaaki Kotera, Nobuhiro Hayashi, Nobutaka Nakashima, Satoshi Nakamura, Shunichiro Ogura, Hideki Taguchi, Masahiro Takinoue, Toshiaki Fukui, Fumio Arisaka, Yasunori Tanji, Hirokazu Urabe, Hisakazu Mihara, Yasunori Aizawa, Hiroyuki Ohta, Kumiko Sogawa, Masayuki Yamamura,Takao Terano, Robert F. Whitter, Robert Cox
Veteran: Ryota Fujiwara, Toshiki Hashimoto, Taku Nakayama, Nobuaki Yasuo, Shinya Suzuki, Hiroshi Fujimoto, Sara Ogino, Shunta Suzuki

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-<meta name="keywords" content="" />
+<meta name="keywords" content="iGEM,2014,Tokyo_Tech,Bank E.coli" />
-<meta name="description" content="" />
+<meta name="description" content="iGEM2014 Wiki pages" />
 <meta http-equiv="content-type" content="text/html; charset=utf-8" />
 <title>Tokyo_Tech</title>
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-			<img src="https://static.igem.org/mediawiki/2014/thumb/b/b7/Tokyo_Tech_Logo.png/613px-Tokyo_Tech_Logo.png" height="150" /></h1></div>
-	        <div align="right"><img src="https://static.igem.org/mediawiki/2014/b/b4/Tokyo_Tech_Logo2.png" height="150" /></div>
+		<a href="https://2014.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/thumb/8/84/Tokyo_Tech_iGEM_logo.png/794px-Tokyo_Tech_iGEM_logo.png" width="90" /></a></div>
          </div>
 	<!-- end #header -->
+        <a href="http://www.titech.ac.jp/english/index.html" id="logo-link"></a>
          <div id="menu">
 		<ul>
@@ Line 20: / Line 22: @@
 			<li><a href="#">Experiment</a>
 			   <ul>
-			        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/C4HSL-dependent_3oxoC12HSL_production" style="width:400px; margin-left:-135px;">C4HSL-dependent 3oxoC12HSL production</a></li>
+			        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Plux and Prhl reporter assay" style="width:400px; margin-left:-135px;">Plux and Prhl reporter assay</a></li>
-			        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/3oxoC12HSL-dependent_C4HSL_production" style="width:400px; margin-left:-135px;">3oxoC12HSL-dependent C4HSL production</a></li>
+			        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay" style="width:400px; margin-left:-135px;">Improved Prhl reporter assay</a></li>
-			        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Symbiosis_confirmation_by_co-culture" style="width:400px; margin-left:-135px;">Symbiosis confirmation by co-culture </a></li>
+			        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/C4HSL-dependent_3OC12HSL_production" style="width:400px; margin-left:-135px;">C4HSL-dependent 3OC12HSL production</a></li>
-			        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay" style="width:400px; margin-left:-135px;">Prhl reporter assay </a></li>
+			        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/3OC12HSL-dependent_C4HSL_production" style="width:400px; margin-left:-135px;">3OC12HSL-dependent C4HSL production</a></li>
+			        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Symbiosis_confirmation_by_co-culture" style="width:400px; margin-left:-135px;">Mutualism Confirmation ~Co-culture Assay~</a></li>
 			   </ul>
 			</li>
 			<li><a href="#">Modeling</a>
-                            <ul>
+                           <ul>
-                                <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Modeling1" style="width:260px; margin-left:-63px;">なぞなぞ</a></li>
+                               <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Overview"  style="width:400px; margin-left:-135px;">Overview</a></li>
-                                <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Modeling2" style="width:260px; margin-left:-63px;">Parameter sensitivity analysis</a></li>
+                               <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Growth Conditions For Company And Customer"  style="width:400px; margin-left:-135px;">Growth Conditions For Company And Customer</a></li>
-                                <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Modeling3" style="width:260px; margin-left:-63px;">The effect of economic wave</a></li>
+                               <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Analysis of C4HSL-dependent Switch" style="width:400px; margin-left:-135px;">Analysis of C4HSL-dependent Switch</a></li>
-                            </ul>
+                               <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Economic Wave"  style="width:400px; margin-left:-135px;">Economic Wave</a></li>
+                           </ul>
                          </li>
 			<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">Parts</a></li>
-			<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices" style="height:45px; padding-top:4px;">Policy and Practices</a></li>
+			<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices" style="height:50px; padding-top:3px;">Policy and Practices</a></li>
 			<li class="current_page_item"><a href="#">More</a>
 			    <ul>
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-            <div class="post">
+        <div class="post">
-	       <h2 class="title"><a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practice">Attribution and Contribution </a></h2>
+          <h2 class="title">Attribution and Contribution</h2>
-              <span class="meta">We could not reach this point without everyone </span>
+              <span class="meta">We could not reach this point without anyone </span>
               <div class="entry-long">
+               <div id="gototop"><a href="#"><img src="https://static.igem.org/mediawiki/2014/5/55/Tokyo_Tech_Go-to-top-icon.png" height="50" /></a></div>
                 <table width="900" border="0">
                   <tr>
@@ Line 78: / Line 84: @@
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">We are proud to say that all the ideas we worked on this summer were proposed by undergraduate members of our team Tokyo_Tech 2014. Moreover, the whole experimental data created by our team comes from experiments all carried out by undergraduates. The "Bank E. coli" project was proposed by Naoto Takahashi. Modeling related to this project was done by Takuya Kato and Ye Kai. We want to thank every advisors and instructors who helped us discussing these ideas and guided us in the experiments.</td>
+                    <td colspan="2"><p class="info-18">We are proud to say that all the ideas we worked on this year were proposed by undergraduate members of our team Tokyo_Tech 2014. Moreover, the whole experimental data created by our team comes from experiments all carried out by undergraduates. The project "Bank <i>E. coli</i>" was proposed by Naoto Takahashi. Modeling related to this project was done by Takuya Kato and Ye Kai. We want to thank every advisers and instructors who helped us with discussing these ideas and guided us in the experiments.</p></td>
                   </tr>
                   <tr>
@@ Line 85: / Line 91: @@
                   </tr>
                   <tr>
-                   <td class="head">Student team members </td>
+<td class="head">Student team members </td>
                     <td>&nbsp;</td>
                   </tr>
                   <tr>
                     <td colspan="2">&nbsp;</td>
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">Performing all  cloning (transformation, culture cells, mini prep):</td>
+                    <td colspan="2" class="info-18">All cloning (transformation, culture cells, mini prep):</td>
                   </tr>
-               <tr>
+                 <tr>
-                    <td colspan="2" class="info">     -
+                    <td colspan="2"><p class="info">     - Yuta Kino, Yuya Nakamura, Kohdai Hibi with helping of other team members </p>                   </td>
-                     <em>Miyabi Hishinuma</em>, <em>Ryu Gyomon, Xinan Wang</em></td>
-                 </tr>
-             <tr>
-                   <td colspan="2" class="info">     -
-                     <em>Miyabi Hishinuma</em>, <em>Ryu Gyomon, Xinan Wang</em></td>
                   </tr>
                   <tr>
@@ Line 106: / Line 108: @@
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">Performing Bank <em>E. coli</em> assay: </td>
+                    <td colspan="2" class="info-18">Parts construction (restriction digest, ligation):</td>
                   </tr>
                   <tr>
-                    <td colspan="2" class="info">     -
+                    <td colspan="2"><p class="info">     - Yuta Kino, Yuya Nakamura, Kohdai Hibi </p></td>
-                     <em>Miyabi Hishinuma</em>, <em>Ryu Gyomon, Xinan Wang</em></td>
                   </tr>
                   <tr>
@@ Line 116: / Line 117: @@
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">Performing Fertilizer assay  (experiment using DAPI, Malachite Green): </td>
+                    <td colspan="2" class="info-18">AHL-dependent gene expression assay: </td>
                   </tr>
                   <tr>
-                    <td colspan="2"><ul class="info">
+                    <td colspan="2"><p class="info">    - Shoko Suzuki, Naoto Takahashi, Keina Matsumura</p></td>
-                       <li>     -
-                         <em>Shoko Suzuki, </em><em>Keina Matsumura, Ay</em><em>aka Murase</em></li>
-                   </ul></td>
                   </tr>
                   <tr>
@@ Line 128: / Line 127: @@
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">Performing parts construction  (restriction digest, ligation): </td>
+                    <td colspan="2" class="info-18">Co-culture assay: </td>
                   </tr>
                   <tr>
-                    <td colspan="2"><ul class="info">
+                    <td colspan="2"><p class="info">    - Yuya Nakamura, Gyomon Ryu</p></td>
-                       <li>     -
-                         <em>Yuta Kino</em>,
-                     <em>Yuya Nakamura, </em><em>Kohdai Hibi</em></li>
-                   </ul></td>
                   </tr>
                   <tr>
@@ Line 141: / Line 136: @@
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">Modeling (simulation of the  genetic circuit)</td>
+                    <td colspan="2" class="info-18">Improved rhl promoter assay: </td>
                   </tr>
                   <tr>
-                    <td colspan="2" class="info">     -
+                    <td colspan="2"><p class="info">    - Naoto Takahashi, Keina Matsumura, Ayaka Murase </p></td>
-                     <em>Takuya Kato,</em>
-                   <em>Ye Kai</em> </td>
                   </tr>
                   <tr>
@@ Line 152: / Line 145: @@
                   </tr>
                   <tr>
-                    <td colspan="2"><div align="center" class="title-small"> Contribution</div></td>
+                    <td colspan="2" class="info-18">Modeling (simulation of the genetic circuit):</td>
                   </tr>
                   <tr>
-                    <td colspan="2">&nbsp;</td>
+                    <td colspan="2" class="info"><p class="info">     - Takuya Kato, Kai Ye </p></td>
                   </tr>
                   <tr>
-                    <td colspan="2" class="head">General Support</td>
+                    <td colspan="2">&nbsp;</td>
                   </tr>
                   <tr>
-                    <td colspan="2">&nbsp;</td>
+                    <td colspan="2" class="info-18">Wiki and slide design: </td>
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">- Prof. Kiga let us use his lab for all  of our experiments. He also has led many Tokyo Tech teams to the excellent  results in iGEM since this competition has started.</td>
+                    <td colspan="2" class="info"><p class="info">     - Paniti Achararit, Kai Ye, Riku Shinohara </p></td>
                   </tr>
                   <tr>
@@ Line 170: / Line 163: @@
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">-  Prof. Yamamura and Prof. Konagaya supported us in many ways to give us a better  researching environment.&nbsp; </td>
+                    <td colspan="2" class="info-18">Policy and Practices: </td>
+                 </tr>
+                 <tr>
+                   <td colspan="2" class="info"><p class="info">     - Miyabi Hishinuma, Ayaka Murase with helping of other team members </p></td>
                   </tr>
                   <tr>
@@ Line 176: / Line 172: @@
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">- Prof. Ayukawa supported us in our experiment throughout the preparations for  iGEM.</td>
+                    <td colspan="2"><div align="center" class="title-small">Contribution</div></td>
                   </tr>
                   <tr>
@@ Line 182: / Line 178: @@
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">- Mr. Sekine and Mr. Amikura gave us  the helpful advice for playing an active part in iGEM competition.&nbsp;</td>
+                    <td colspan="2" class="head">General Support</td>
                   </tr>
                   <tr>
@@ Line 188: / Line 184: @@
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">- Promega provided us the part of restriction enzyme we used without any charge.</td>
+                    <td colspan="2" class="info-18">Prof. Kiga let us use his lab for all of our experiments. He also has led many Tokyo Tech teams to the excellent results in iGEM since this competition has started.</td>
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">&nbsp;</td>
+                    <td colspan="2" class="info-18">Prof. Yamamura and Prof. Konagaya supported us in many ways to give us a better researching environment.&nbsp; </td>
                   </tr>
                   <tr>
-                    <td colspan="2" class="head">Instrumental Analysis Support</td>
+                    <td colspan="2" class="info-18">Prof. Ayukawa supported us in all of our experiments throughout the preparations for iGEM.
+                   </td>
                   </tr>
                   <tr>
-                   <td colspan="2">&nbsp;</td>
+                    <td colspan="2" class="info-18">Promega KK provided us a part of the restriction enzyme we used without any charge.
-                 </tr>
+                   </td>
-                 <tr>
-                    <td colspan="2" class="info-18">- Biomaterial Analysis  Center, Technical Department, sequenced the sequence of plasmids which we had  asked for. </td>
                   </tr>
                   <tr>
                     <td colspan="2" class="info-18">&nbsp;</td>
                   </tr>
                   <tr>
-                    <td colspan="2" class="head">Human Practice Support</td>
+                    <td colspan="2" class="head">Instrumental Analysis Support</td>
                   </tr>
                   <tr>
@@ Line 212: / Line 210: @@
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">- Mr. Katayama who is working in Tokyo Institute of Technology Graduate  School of Innovation Management gave us his opinion on issues of environmental  and beneficiary survey of our projects.</td>
+                    <td colspan="2" class="info-18">Biomaterial Analysis Center, Technical Department, checked the sequence of the plasmids which we had asked. </td>
                   </tr>
                   <tr>
-                    <td colspan="2">&nbsp;</td>
+                    <td colspan="2" class="info-18">&nbsp;</td>
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">-
+                    <td colspan="2" class="head">Policy and Practice Support</td>
-Mr. Kawabata who is working in JA ZENNOH CHIBA advised what problems and benefits  will be occurred if our project was applied.</td>
                   </tr>
                   <tr>
@@ Line 225: / Line 222: @@
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">-
+                    <td colspan="2" class="info-18">Tomoya Saito, who is working for National Institute of Public Health, gave us a lecture on dual-use technologies, and gave us useful advice about <a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices/Our_question_about_biosafety">biosafety.</a></td>
-Takeda Science Foundation introduced us in their book.</td>
                   </tr>
                   <tr>
-                    <td colspan="2">&nbsp;</td>
+                    <td colspan="2" class="info-18">Hiromichi Ando arranged <a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices#MUSE">MUSE TALK</a> and let us present there.</td>
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">-
+                    <td colspan="2" class="info-18">Akihiro Hiyoshi, who is the principal of <a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices#Visit">Ohyamaguchi Elementary School</a>, gave us a chance to communicate with students.</td>
-Mr. Sato (a teacher of Shinjuku high school) proposed the experimental classroom, and led us.</td>
+                  </tr>
-                  </tr>
                   <tr>
-                    <td colspan="2">&nbsp;</td>
+                    <td colspan="2" class="info-18">&nbsp;</td>
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">-
+                    <td colspan="2" class="head">Presentation Adviser</td>
-Ms. Inoue (a teacher of Sagamihara high school) and Ms. Miyamoto (a teacher of  Toin Gakuen) collected lots of high school students to our experimental classroom.</td>
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">&nbsp;</td>
+                    <td colspan="2">&nbsp;</td>
                   </tr>
                   <tr>
-                    <td colspan="2" class="head">And last but not least</td>
+                    <td colspan="2" class="info-18">We would like to thank all the people who came to check our presentations and gave us useful feedbacks:</td>
                   </tr>
                   <tr>
-                    <td colspan="2">&nbsp;</td>
+                    <td colspan="2"><p class="info"><strong>Professor: </strong>Masaaki Wachi, Hiroshi Iwasaki, Masaaki Kotera, Nobuhiro Hayashi, Nobutaka Nakashima, Satoshi Nakamura, Shunichiro Ogura, Hideki Taguchi, Masahiro Takinoue, Toshiaki Fukui, Fumio Arisaka, Yasunori Tanji, Hirokazu Urabe, Hisakazu Mihara, Yasunori Aizawa, Hiroyuki Ohta, Kumiko Sogawa, Masayuki Yamamura,Takao Terano, Robert F. Whitter, Robert Cox</td>
                   </tr>
                   <tr>
-                    <td colspan="2" class="info-18">We would like to thank everyone who came to our  progress presentations and gave useful feedback and input:</td>
+                    <td colspan="2"><p class="info"><strong>Veteran: </strong>Ryota Fujiwara, Toshiki Hashimoto, Taku Nakayama, Nobuaki Yasuo, Shinya Suzuki, Hiroshi Fujimoto, Sara Ogino, Shunta Suzuki</td>
-                 </tr>
-                 <tr>
-                   <td colspan="2" class="info">          - <em>Fumio Arisaka, Toshiaki Fukui, Takeshi Hata, Junji  Hirota, Hiroshi Iwasaki, Satoshi Nakamura, Shunichiro Ogura, Hideki Taguchi,  Masahiro Takinoue, Yasunori Tanji, Hirokazu Urabe, Masaaki Wachi, Robert F.  Whittier, Daniel Berrar, Mai Miura, Taku Nakayama, Goshi Sugano, Naoki Tsukuda  and Nobuaki Yasuo</em></td>
                   </tr>
                 </table>
                 <p>&nbsp;</p>
-              </div>
+          </div>
-            </div>
+        </div>
 	  </div>
 		<!-- end #content -->