Team:Tokyo Tech

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<title>Tokyo_Tech</title>
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<li><a href="#">Experiment</a>
<li><a href="#">Experiment</a>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/C4HSL-dependent_3oxoC12HSL_production" style="width:400px; margin-left:-135px;">C4HSL-dependent 3oxoC12HSL production</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Plux and Prhl reporter assay" style="width:400px; margin-left:-135px;">Plux and Prhl reporter assay</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/3oxoC12HSL-dependent_C4HSL_production" style="width:400px; margin-left:-135px;">3oxoC12HSL-dependent C4HSL production</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay" style="width:400px; margin-left:-135px;">Improved Prhl reporter assay</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Symbiosis_confirmation_by_co-culture" style="width:400px; margin-left:-135px;">Symbiosis confirmation by co-culture </a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/C4HSL-dependent_3OC12HSL_production" style="width:400px; margin-left:-135px;">C4HSL-dependent 3OC12HSL production</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay" style="width:400px; margin-left:-135px;">Prhl reporter assay </a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/3OC12HSL-dependent_C4HSL_production" style="width:400px; margin-left:-135px;">3OC12HSL-dependent C4HSL production</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Symbiosis_confirmation_by_co-culture" style="width:400px; margin-left:-135px;">Mutualism Confirmation ~Co-culture Assay~</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling">Modeling</a></li>
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<li><a href="#">Modeling</a>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Overview"  style="width:400px; margin-left:-135px;">Overview</a></li>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Growth Conditions For Company And Customer"  style="width:400px; margin-left:-135px;">Growth Conditions For Company And Customer</a></li>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Analysis of C4HSL-dependent Switch" style="width:400px; margin-left:-135px;">Analysis of C4HSL-dependent Switch</a></li>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Economic Wave"  style="width:400px; margin-left:-135px;">Economic Wave</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">Parts</a></li>
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">Parts</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices" style="height:45px; padding-top:4px;">Policy and Practices</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices" style="height:50px; padding-top:3px;">Policy and Practices</a></li>
<li><a href="#">More</a>
<li><a href="#">More</a>
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<h2 class="title">Welcome</h2>
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            <span class="meta">This is iGEM Tokyo_Tech 2014 Wiki!</span>
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    <a href="http://www.titech.ac.jp/english/index.html"><img src="https://static.igem.org/mediawiki/2014/b/b7/Tokyo_Tech_Logo.png" height="300"/></a>
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    <a href="http://www.titech.ac.jp/english/index.html"><p> Tokyo Institute of Technology </p></a>
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    <a href="https://2014.igem.org/Team:Tokyo_Tech/Project"><img src="https://static.igem.org/mediawiki/2014/3/3e/Tokyo_Tech_Bank_State.png" height="300"/></a>
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    <a href="https://2014.igem.org/Team:Tokyo_Tech/Project"><p> Project </p></a>
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    <a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices"><img src="https://static.igem.org/mediawiki/2014/thumb/4/49/Tokyo_Tech_Poster_session1.jpg/800px-Tokyo_Tech_Poster_session1.jpg" height="290"/></a>
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    <a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices"><p> Policy and Practices </p></a>
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    <a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices#Inheritance"><img src="https://static.igem.org/mediawiki/2014/thumb/2/23/Tokyo_Tech_Congress_Group_Photo.jpg/800px-Tokyo_Tech_Congress_Group_Photo.jpg" height="250" /></a>
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    <a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices#Inheritance"><p> Genetics Society of Japan </p></a>
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                         <td colspan="2"><h2>Project background</h2></td>
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                         <td colspan="2"><h2>Project Planning</h2></td>
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                         <td width="450" rowspan="3"><p align="justify" class="info-18">We have learned a lot about life  science in Tokyo Tech. However, through “policy and practice” interaction to  general public people at early stage of our project plannings (Fig. 1), we noticed that we don’t know what economics is like in detail. Therefore, we decided  to create an educational tool showing the economics by using E. coli, our  familiar XXX. We think that an educational tool using E. coli will be perfect  for iGEMers.</p></td>
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                         <td width="450" rowspan="3"><p align="justify" class="info-18">Through interaction with the general public people in our project planning stage, we realized that we do not know much about economics in spite of our study for biology.</p>
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                        <p align="justify" class="info-18">For our future innovation based on knowledge of economics, we thought of creating an educational tool of economics by using <i>E. coli</i> and BioBrick, which are familiar for iGEMers.</p></td>
                         <td width="450" height="253"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Poster_session2.jpg"><img src="https://static.igem.org/mediawiki/2014/thumb/6/60/Tokyo_Tech_Poster_session2.jpg/800px-Tokyo_Tech_Poster_session2.jpg" alt="" width="380" /></a></div></td>
                         <td width="450" height="253"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Poster_session2.jpg"><img src="https://static.igem.org/mediawiki/2014/thumb/6/60/Tokyo_Tech_Poster_session2.jpg/800px-Tokyo_Tech_Poster_session2.jpg" alt="" width="380" /></a></div></td>
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                         <td><div align="center">Fig.1-1-1 Appearance of  poster session</div></td>
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                         <td><div align="center"><strong>Fig. 1-1-1.</strong> Our members discussing about the project with the visitor</div></td>
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                         <td>&nbsp;</td>
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                        <td height="23" colspan="2"><div align="right" class="info-18">(<a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices">Go to Policy and Practices Page</a>)</div></td>
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                         <td colspan="2"><h2>Story</h2></td>
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                         <td colspan="2"><h2>Project Overview</h2></td>
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                         <td rowspan="3"><p align="justify" class="info-18">Our educational tool for economics has three types  of E. coli: Bank, Company, and Customer. (Fig. 2).  Like the exchange of money and products in the real economy, we make these E. coli exchange products and money in this system. Company makes products, and sell them to customers. Customer pays money for the products made by  Company. Bank regulates  money supply. 中央銀行FRBやXXやXXを示しているよ。⇒That means FRS, or a  certain<strong> </strong><a href="http://ejje.weblio.jp/content/hamburger" title="hamburgerの意味">hamburger</a>&nbsp;<a href="http://ejje.weblio.jp/content/franchise" title="franchiseの意味">franchise</a>. Here, signal molecule of the quorum sensing.  3OC12HSL and C4HSL represent products and money.</p></td>
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                         <td rowspan="3"><p class="info-18">Like the exchange of money and products in the real economy, we designed three types of <i>E.coli</i> (Bank, Company, and Customer) which exchange Product and Money in the tool&rsquo;s economic system. Product and Money were represented by 3OC12HSL and C4HSL, the signaling molecules of the quorum sensing, respectively.  In the final step of our wet experiments, we achieved the mutualism of Company and Customer in test tubes.</p><p align="justify" class="info-18">&nbsp;</p></td>
                         <td height="227"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_1-1-2.png"><img src="https://static.igem.org/mediawiki/2014/0/09/Tokyo_Tech_1-1-2.png" alt="" width="450" /></a></div></td>
                         <td height="227"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_1-1-2.png"><img src="https://static.igem.org/mediawiki/2014/0/09/Tokyo_Tech_1-1-2.png" alt="" width="450" /></a></div></td>
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                         <td><div align="center">Fig.1-1-2 Relationship among three type of E.coli</div></td>
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                         <td><div align="center"><strong>Fig. 1-1-2.</strong> Relationship among three types of <i>E. coli</i></div></td>
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                         <td>&nbsp;</td>
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                         <td><div align="right" class="info-18">(<a href="https://2014.igem.org/Team:Tokyo_Tech/Project">Go to Project Page</a>)</div></td>
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                          <p><a href="https://2014.igem.org/File:Tokyo_Tech_Fig1-1-3.png"><img src="https://static.igem.org/mediawiki/2014/5/54/Tokyo_Tech_Fig1-1-3.png" width="300" height="300" /></a></p>
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                        <strong>Fig. 1-1-3.</strong> The growth dependency of Prhl and Plux promoters </div></td>
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                        <td width="529" rowspan="3"><p><span class="info-18">From the simulation before wet experiments, we noticed that the strength of the two signaling-molecule dependent promoters, Prhl and Plux, need to be close in order to promote Company and Customer&rsquo;s growth. However, in our first step of the wet experiments, Prhl promoter showed far less fluorescence intensity than Plux promoter<a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay).Thus">.</a></span>
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                          </p>
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                          <p align="right" class="info-18">(<a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Plux_and_Prhl_reporter_assay">Go to Plux and Prhl reporter assay page</a>)</p>
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                         <td colspan="2"><div align="right" class="info-18"></div></td>
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                        <td rowspan="3"><p class="info-18">Company and Customer have an interdependence by using  two types of cell-cell commutation molecules:C4 and C12. 簡潔すぎThe results of co-culture experiment is Fig. 3. &nbsp;共培養でXXXが分かる&nbsp;</p></td>
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                         <td height="19"><div align="center"> Fig.1-1-3 The result of co-culture assay</div></td>
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                         <td height="434"><div align="center">
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                          <p align="left" class="info-18">Thus, we improved the promoters fluorescence intensity by recombining regulatory-protein binding sites. Our Prhl(RL) promoter (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1529300">K1529300</a>), which has the highest S/N ratio and much higher activity than native Prhl promoter, was used for the following experiments.&nbsp;</p>
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                          <p align="right" class="info-18">&nbsp;</p>
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                          <p align="left" class="info-18">&nbsp;</p>
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                        <td><p><a href="https://2014.igem.org/File:Tokyo_Tech_Fig1-1-4.png"><img src="https://static.igem.org/mediawiki/2014/d/d9/Tokyo_Tech_Fig1-1-4.png" width="541" height="283" /></a></p>
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                          <div align="center"> <strong>Fig. 1-1-4.</strong> Our improved Prhl promoter                         
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                            <p align="right">&nbsp;</p>
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                            <p align="right"><span class="info-18">(<a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay">Go to Prhl reporter assay page</a>)</span></p>
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                         <td width="413"><p class="info-18">We then demonstrated the mutualism between Company and Customer through modeling and wet-experiments.  By measuring the fluorescence intensity of GFP with flow cytometer, the co-culture of the Company and Customer shows that company and customer promoted the growth of each other (Fig. 1-1-5).</p></td>
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                        <td width="477"><p align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Fig1-1-5.png"><img src="https://static.igem.org/mediawiki/2014/1/16/Tokyo_Tech_Fig1-1-5.png" width="445"  /></a></p>
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                        <div align="center"><strong>Fig. 1-1-5.</strong> Company and Customer co-culture assay</div></td>
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                         <td rowspan="3"><p align="justify" class="info-18">Compared  with the case of single  culture&nbsp;,  coexistence with  &nbsp;Company  and Customer in one test tube grow better. Modeling&nbsp; (Fig.  4) shows the same results to the wet experiment.we show  the same results to the experiment. Mathematical  modeling also shows this interdependent promotion of growth.</p>
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                            <div> </div>
+
-
                        </div></td>
+
-
                        <td height="131"><img src="http://sg.openrice.com/images/v4/previewimg/sr1-icon-noResult.png" alt="" width="450" /></td>
+
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td height="19"><div align="center">Fig.1-1-4 The modeling  of co-culture in large scale</div></td>
+
                         <td colspan="2">&nbsp;</td>
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td height="19">&nbsp;</td>
+
                         <td colspan="2"><div align="right"><span class="info-18">(<a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Symbiosis_confirmation_by_co-culture">Go to Symbiosis Confirmation Co-culture Assay Page</a>)</span></div></td>
                       </tr>
                       </tr>
-
                     
 
                       <tr>
                       <tr>
-
                         <td><img src="http://sg.openrice.com/images/v4/previewimg/sr1-icon-noResult.png" alt="" width="450" /></td>
+
                         <td colspan="2"><div align="center"></div></td>
-
                        <td rowspan="3"><p align="justify" class="info-18">However, in the real economy, there isn’t only one  but many companies&nbsp;. In  other words, money and  products have traded in a small scale.&nbsp; Therefore, we thought we also should trade money and  product in a smaller scale.<u> </u>In modeling (Fig. 5), as the scale become  small, Company and Customer can't keep interdependent. So we should improve our  system/solve this problem.                        </p></td>
+
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td><div align="center"> Fig.1-1-5 The modeling of co-culture
+
                         <td colspan="2">&nbsp;</td>
-
                            in  small scale</div></td>
+
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td height="19">&nbsp;</td>
+
                         <td colspan="2"><p class="info-18">We then introduced Bank to our educational tool.  In the real economy, thousands of Company-Customer pairs exist in a market.  In the emulation of such situation, a pair of Company-Customer is allowed to occupy just small scale.  Even though a large scale economy achieved growth of both Company and Customer (Fig. 1-1-6-A), our modeling results for mutualism in a small scale showed that both company and customer will die at a low initial cell concentration (Fig 1-1-6-B.). In order to solve this problem, we introduced <i>E. coli</i> which functions as a central bank. This Bank <em><i>E. coli</i></em> to the small-scale economy recovers growth of both Company and Customer (Fig 1-1-6-C.).</p></td>
                       </tr>
                       </tr>
-
</table>
 
-
<table width="900" border="0">
 
                       <tr>
                       <tr>
-
                         <td colspan="2" class="head">We made two solutions to this problem</td>
+
                         <td colspan="2"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Fig1-1-6.png"><img src="https://static.igem.org/mediawiki/2014/1/1a/Tokyo_Tech_Fig1-1-6.png" width="730" height="275" /></a></div></td>
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td width="640">&nbsp;</td>
+
                         <td colspan="2"><div align="center"><strong>Fig. 1-1-6.</strong> Bank regulates the money supply</div></td>
-
                        <td width="250">&nbsp;</td>
+
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td rowspan="2"><p class="info-18"><strong>First</strong>, <u>we changed the level of Prhl&nbsp; .</u>The results of Prhl is Fig. 6. Prhl (RR) was known  as much leaking. So, we made Prhl (RL) which leaks smaller than Prhl (RR).                        </p></td>
+
                         <td colspan="2"><div align="center"></div></td>
-
                        <td><div align="center"><img src="http://sg.openrice.com/images/v4/previewimg/sr1-icon-noResult.png" alt="" width="300" /></div></td>
+
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td><div align="center">Fig.1-1-6 The result of  Prhl assay</div></td>
+
                         <td colspan="2">&nbsp;</td>
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td>&nbsp;</td>
+
                         <td width="532"><p class="info-18">In further dense interaction with entrepreneurs, we were pointed out that economic wave should be integrated into our system to make it more realistic. We thus performed a modeling on economic wave to see how it works. Although Economic wave can destroy mutualism system (Fig1-1-8 A,B, D,E), bank allows the system to keep a coexistence relationship (Fig1-1-8 C and F) even when there is a change in the amount of money supply in the market(Fig1-1-8.).</p></td>
-
                         <td>&nbsp;</td>
+
                         <td width="358"><div align="center">
 +
                          <p><a href="https://2014.igem.org/File:Tokyo_Tech_Fig1-1-7.png"><img src="https://static.igem.org/mediawiki/2014/9/95/Tokyo_Tech_Fig1-1-7.png" width="314" height="205" /></a></p>
 +
                          <strong>Fig. 1-1-7.</strong> Our teammates consulting the professionals at the workshop MUSE TALK                        </div></td>
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td rowspan="4"><p align="justify" class="info-18"><strong>Second</strong>, we introduced Bank which regulates money  (C4HSL) supply into our system Depending on the concentration of money  (C4HSL), Bank switches to “collection state”  and “distribution state”. In the collection state, bank decomposes money  (C4HSL) and product (3OC12HSL). In distribution state, bank produces money  (C4HSL) and releases it to market, which is the liquid medium in our  experiments. In modeling (Fig. 7), bank helps the growth of Company and  Customer.</p></td>
+
                         <td colspan="2"><div align="right" class="info-18">(<a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices#MUSE">Go to MUSE TALK Page</a>)</div></td>
-
                        <td height="24">&nbsp;</td>
+
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td height="8"><img src="http://sg.openrice.com/images/v4/previewimg/sr1-icon-noResult.png" alt="" width="300" /></td>
+
                         <td colspan="2">&nbsp;</td>
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td height="3"><div align="center">Fig.1-1-7 The modeling of <br />
+
                         <td colspan="2">&nbsp;</td>
-
                        co-culture including  Bank</div></td>
+
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td height="4">&nbsp;</td>
+
                         <td colspan="2"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Fig1-1-8.png"><img src="https://static.igem.org/mediawiki/2014/0/0f/Tokyo_Tech_Fig1-1-8.png" width="800" height="605" /></a></div></td>
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td>&nbsp;</td>
+
                         <td colspan="2"><div align="center"><strong>Fig. 1-1-8.</strong> Economic wave</div></td>
-
                        <td>&nbsp;</td>
+
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td colspan="2"><p class="info-18">Thanks to advice from people in 企業社会, we  also introduced economic wave into this
+
                         <td colspan="2">&nbsp;</td>
-
                        project  to show more authenticity. <u>We designed money (C12HSL) supply in external  economy.</u> In modeling (Fig. 8), even if there are economic wave, Bank helps  the growth of Company and Customer.</p></td>
+
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td>&nbsp;</td>
+
                         <td colspan="2" class="entry-long">&nbsp;</td>
-
                        <td>&nbsp;</td>
+
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td colspan="2"><div align="center"><img src="http://sg.openrice.com/images/v4/previewimg/sr1-icon-noResult.png" alt="" width="500" /></div></td>
+
                         <td colspan="2">&nbsp;</td>
                       </tr>
                       </tr>
 +
                  </table>
 +
                      <table width="900" border="0">
                       <tr>
                       <tr>
-
                         <td colspan="2"><div align="center"> Fig.1-1-8 The modeling of co-culture
+
                         <td colspan="2"><h2>Project Evaluation</h2></td>
-
                        with economic  wave</div></td>
+
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td>&nbsp;</td>
+
                         <td colspan="2">&nbsp;</td>
-
                        <td>&nbsp;</td>
+
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td><h2>Future Works </h2></td>
+
                         <td colspan="2"><p align="left" class="info-18">Finally, the evaluation of our educational tool using E. coli and
-
                        <td>&nbsp;</td>
+
BioBrick showed that our project is highly effective for students majoring in biology to understand economics (Fig. 1-1-9).</p></td>
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td colspan="2"><p class="info-18">Our big dream is to humanize E. coli. By using E. coli  behaving as if it has the humanity, we are eager to reproduce the whole human  society inside the test tubes. For the first step, we put an eye on money,  which is essential for our lives. By sending and receiving signals as money  circulation, we recreated the system of economy. Although economy was chosen  for our target in this project, we believe we can recreate any human dramas,  such as E. coli falling in love or becoming a liar, inside the test tubes as  well in the near future.</p></td>
+
                         <td colspan="2">&nbsp;</td>
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td>&nbsp;</td>
+
                         <td colspan="2"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Fig1-1-9.png"><img src="https://static.igem.org/mediawiki/2014/a/a0/Tokyo_Tech_Fig1-1-9.png" width="615" height="243" /></a></div></td>
-
                        <td>&nbsp;</td>
+
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td colspan="2"><p class="info-18">In our project, Customer E. coli and Company E. coli are  always threatened of Cm. They cannot live without Bank E. coli which modifies  C4HSL and C12HSL, since the CmR resistance gene cannot be expressed without  these signals. That depicts not only the common capitalistic rule “Bank  modifies the giving and taking between Customer and Company”, but also  recreates the human nature. </p></td>
+
                         <td colspan="2"><div align="center"><strong>Fig. 1-1-9. </strong>Effects of our presentation</div></td>
                       </tr>
                       </tr>
                       <tr>
                       <tr>
Line 209: Line 277:
                       </tr>
                       </tr>
                       <tr>
                       <tr>
-
                         <td colspan="2"><p class="info-18">Man cannot live without others. We can live only when we  remain some relationship with people. The dramatic story of our three E. coli  (Bank, Customer, and Company) derives from this Information-processing system  to recreate our humanity, too.</p></td>
+
                        <td colspan="2"><div align="right" class="info-18">(<a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices">Go to Policy and Practices Page</a>)</div></td>
 +
                      </tr>
 +
                      <tr>
 +
                        <td colspan="2">&nbsp;</td>
 +
                      </tr>
 +
                      <tr>
 +
                        <td colspan="2">&nbsp;</td>
 +
                      </tr>
 +
                      <tr>
 +
                        <td colspan="2" class="entry-long">&nbsp;</td>
 +
                      </tr>
 +
                      <tr>
 +
                        <td colspan="2">&nbsp;</td>
 +
                      </tr>
 +
                      <tr>
 +
                        <td colspan="2"><h2>Future Works </h2></td>
 +
                      </tr>
 +
                      <tr>
 +
                         <td colspan="2"><p align="left" class="info-18">Our big dream is to allow <i>E. coli</i> to obtain &quot;sociability&quot;. By using more signaling molecules and other types of materials, we are eager to simulate the whole human society by building a larger network in test tubes. Moreover, our achievements can be applied to multistage substance producing. Microbial consortia can perform even more complicated tasks through the division of labor by using the mutuality. For such construction of consortia, the improved Prhl promoter broadens quorum sensing, and updates the previous cell-cell communication systems greatly.</p><p class="info-18">&nbsp;</p></td>
                       </tr>
                       </tr>
-
                    </table>
+
            </table>
-
<p>&nbsp;</p>
+
    <table width="900" border="0">
 +
    <tbody>
 +
      <tr>
 +
        <td class="entry-long">&nbsp;</td>
 +
          </tr>
 +
      <tr>
 +
        <td>&nbsp;</td>
 +
          </tr>
 +
      <tr>
 +
        <td><h2>Sponsors</h2></td>
 +
          </tr>
 +
      <tr>
 +
        <td>&nbsp;</td>
 +
          </tr>
 +
      <tr>
 +
        <td><p align="center"><img src="https://static.igem.org/mediawiki/2014/thumb/4/4b/Tokyo_Tech_Cosmobio.png/120px-Tokyo_Tech_Cosmobio.png" alt="" width="160" height="160"><img src="https://static.igem.org/mediawiki/2014/0/07/Tokyo_Tech_Promega.jpg" alt="" width="272" height="172"><img src="https://static.igem.org/mediawiki/2014/f/fe/Tokyo_Tech_IDT.jpg" alt="" width="356" height="148"><img src="https://static.igem.org/mediawiki/2014/a/ab/Titech_Foundation.gif" alt="" width="312" height="143"><img src="https://static.igem.org/mediawiki/2014/0/01/Tokyo_Tech_Space.png" width="30" height="55" /><img src="https://static.igem.org/mediawiki/2014/7/70/Tokyo_Tech_Mbl.png" alt="" width="258" height="124"><img src="https://static.igem.org/mediawiki/2014/c/c7/Tokyo_Tech_JASSO.jpg" alt="" width="445" height="140"><img src="https://static.igem.org/mediawiki/2014/2/2e/Tokyo_Tech_MathWorks.jpg" alt="" width="445" height="88"><img src="https://static.igem.org/mediawiki/2014/4/43/Tokyo_Tech_Leaveanest.png"><img src="https://static.igem.org/mediawiki/2014/0/01/Tokyo_Tech_Space.png" width="20" height="55" /><img src="https://static.igem.org/mediawiki/2014/f/f1/Tokyo_Tech_Alumni_Foundation.gif" alt="" width="353" height="113"><img src="https://static.igem.org/mediawiki/2014/2/2d/Tokyo_Tech_Sponsorsisao.png" width="300" height="55" /><img src="https://static.igem.org/mediawiki/2014/d/d3/Tokyo_Tech_Sponsorsaizawa.png" width="500" height="48" /></p></td>
 +
          </tr>
 +
     
 +
    </table>
 +
    <p>&nbsp;</p>
  </div>
  </div>
    </div>
    </div>

Latest revision as of 03:43, 18 October 2014

Tokyo_Tech

Welcome

This is iGEM Tokyo_Tech 2014 Wiki!
 
 

Project Planning

Through interaction with the general public people in our project planning stage, we realized that we do not know much about economics in spite of our study for biology.

For our future innovation based on knowledge of economics, we thought of creating an educational tool of economics by using E. coli and BioBrick, which are familiar for iGEMers.

Fig. 1-1-1. Our members discussing about the project with the visitor
 
 
 
 

Project Overview

Like the exchange of money and products in the real economy, we designed three types of E.coli (Bank, Company, and Customer) which exchange Product and Money in the tool’s economic system. Product and Money were represented by 3OC12HSL and C4HSL, the signaling molecules of the quorum sensing, respectively.  In the final step of our wet experiments, we achieved the mutualism of Company and Customer in test tubes.

 

Fig. 1-1-2. Relationship among three types of E. coli

Fig. 1-1-3. The growth dependency of Prhl and Plux promoters

From the simulation before wet experiments, we noticed that the strength of the two signaling-molecule dependent promoters, Prhl and Plux, need to be close in order to promote Company and Customer’s growth. However, in our first step of the wet experiments, Prhl promoter showed far less fluorescence intensity than Plux promoter.

(Go to Plux and Prhl reporter assay page)

Thus, we improved the promoters fluorescence intensity by recombining regulatory-protein binding sites. Our Prhl(RL) promoter (K1529300), which has the highest S/N ratio and much higher activity than native Prhl promoter, was used for the following experiments. 

 

 

Fig. 1-1-4. Our improved Prhl promoter

 

(Go to Prhl reporter assay page)

 

We then demonstrated the mutualism between Company and Customer through modeling and wet-experiments.  By measuring the fluorescence intensity of GFP with flow cytometer, the co-culture of the Company and Customer shows that company and customer promoted the growth of each other (Fig. 1-1-5).

Fig. 1-1-5. Company and Customer co-culture assay
 
 
 

We then introduced Bank to our educational tool.  In the real economy, thousands of Company-Customer pairs exist in a market.  In the emulation of such situation, a pair of Company-Customer is allowed to occupy just small scale.  Even though a large scale economy achieved growth of both Company and Customer (Fig. 1-1-6-A), our modeling results for mutualism in a small scale showed that both company and customer will die at a low initial cell concentration (Fig 1-1-6-B.). In order to solve this problem, we introduced E. coli which functions as a central bank. This Bank E. coli to the small-scale economy recovers growth of both Company and Customer (Fig 1-1-6-C.).

Fig. 1-1-6. Bank regulates the money supply
 

In further dense interaction with entrepreneurs, we were pointed out that economic wave should be integrated into our system to make it more realistic. We thus performed a modeling on economic wave to see how it works. Although Economic wave can destroy mutualism system (Fig1-1-8 A,B, D,E), bank allows the system to keep a coexistence relationship (Fig1-1-8 C and F) even when there is a change in the amount of money supply in the market(Fig1-1-8.).

Fig. 1-1-7. Our teammates consulting the professionals at the workshop MUSE TALK
 
 
Fig. 1-1-8. Economic wave
 
 
 

Project Evaluation

 

Finally, the evaluation of our educational tool using E. coli and BioBrick showed that our project is highly effective for students majoring in biology to understand economics (Fig. 1-1-9).

 
Fig. 1-1-9. Effects of our presentation
 
 
 
 
 

Future Works

Our big dream is to allow E. coli to obtain "sociability". By using more signaling molecules and other types of materials, we are eager to simulate the whole human society by building a larger network in test tubes. Moreover, our achievements can be applied to multistage substance producing. Microbial consortia can perform even more complicated tasks through the division of labor by using the mutuality. For such construction of consortia, the improved Prhl promoter broadens quorum sensing, and updates the previous cell-cell communication systems greatly.

 

 
 

Sponsors