Team:Oxford/how much can we degrade
From 2014.igem.org
(Difference between revisions)
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<li>d[Ndcm]/dt = rate of DCM molecule degradation (s-1)</li> | <li>d[Ndcm]/dt = rate of DCM molecule degradation (s-1)</li> | ||
- | <li> | + | <li>kcat = dcmA turnover rate (= 0.6 s-1 for DM4)</li> |
<li>[DCM] = DCM concentration (= 0.02M for our system)</li> | <li>[DCM] = DCM concentration (= 0.02M for our system)</li> | ||
<li>[DcmA] = Number of DcmA molecules per cell (87576) <a href="https://2014.igem.org/Team:Oxford/what_are_microcompartments?#hide4"> | <li>[DcmA] = Number of DcmA molecules per cell (87576) <a href="https://2014.igem.org/Team:Oxford/what_are_microcompartments?#hide4"> | ||
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<div class="row"> | <div class="row"> | ||
<a href="#show6" class="show modelling-row" id="show6"><div class="modelling"> | <a href="#show6" class="show modelling-row" id="show6"><div class="modelling"> | ||
- | <h1white>How does the | + | <h1white>How does the kcat of the system affect the output?</h1white> |
<img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" /> | <img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" /> | ||
</div></a> | </div></a> | ||
<a href="#hide6" class="hide" id="hide6"><div class="modelling"> | <a href="#hide6" class="hide" id="hide6"><div class="modelling"> | ||
- | <h1white>How does the | + | <h1white>How does the kcat of the system affect the output?</h1white></div></a> |
<div class="list"> | <div class="list"> | ||
<div class="white_news_block2"> | <div class="white_news_block2"> | ||
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<br><br> | <br><br> | ||
We used our model to predict the response of the system to a change in the kcat value of the DCM degradation enzyme, dcmA. | We used our model to predict the response of the system to a change in the kcat value of the DCM degradation enzyme, dcmA. | ||
- | Increasing the value of | + | Increasing the value of kcat by a significant amount is unrealistic in the length of our project. However, in future work, the kcat could potentially be substantially improved. |
<br><br> | <br><br> | ||
In the graph shown here, the total volume degraded doesn't change. This is because the amount of HCl that the system requires to reach a toxic pH level is constant, as we are not varying the volume of the aqueous layer. To increase the total amount of DCM degraded, we simply need to add more water or a pH buffer to the system. | In the graph shown here, the total volume degraded doesn't change. This is because the amount of HCl that the system requires to reach a toxic pH level is constant, as we are not varying the volume of the aqueous layer. To increase the total amount of DCM degraded, we simply need to add more water or a pH buffer to the system. |
Revision as of 00:00, 18 October 2014