Team:Evry/Overview/Achievements

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<h1 color=#000033 >The Sponge Patrol Project</h1>
 
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For the third participation to the iGEM competition, we decided to introduce a new chassis to the competition: <i>Pseudovibrio denitrificans</i>.
 
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Sum up of overview parts
 
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<br>Le détail des 3 soius-unités avec une image sympa pour chacun, on clique dessus pour avoir la suite
 
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Chassis :  draw a bacteria in water the same as the one in the logo
 
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<h4 > A specific chassis for a specific environment: <br>  Seas & Oceans </h4>
 
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As seen in the <a href="https://2014.igem.org/Team:Evry/Overview" <b><big> overview</big></b></a> our biosensors should work optimally in native marine conditions, a conditions for which <b><font color= "red"> no current chassis</font></b> in iGEM is ready for.
 
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Moreover it has to attach to sponges and stick with them without disturbing the microbiome for as long as possible.
 
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Therefore came up the necessity to use a bacterium naturally present on the sponges, may it be an epibiont or a symbiont.
 
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<h5 > Bacterium properties: </h4>
 
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<li> Must be massively present on the sponge surface : <font color=#6699CC>avoid being in an unfavourable position for food competition. </font> </li>
 
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<li> Must be found mainly in sponges : <font color=#6699CC>avoid spreading to species in contact with sponges.</font> </li>
 
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<li> Must be the phenotypically closest possible  to a known bacterium : <font color=#6699CC>avoid cell cultures difficulties.</font> </li>
 
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<h5>Sponge properties:</h4>
 
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<li> Must be numerously present in the ocean : <font color=#6699CC>avoiding putting species in danger.</font> </li>
 
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<li> Must be a natural host of the bacterium : <font color=#6699CC>avoid adapting its microbiom to the new epibiont.</font> </li>
 
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<li> Must be easily culturable in laboratoty : <font color=#6699CC> avoid sponge culture difficulties.</font> </li>
 
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The closest combination sponge/bacterium that could fit the requirement is <i><big>Spongia Officinalis / Pseudovibrio denitrificans </big></i>
 
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Hence, <i>Pseudovibrio denitrificans </i> living in symbiosis with the sponge : Spongia Officinalis.
 
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<a href="https://2014.igem.org/Team:Evry/Biology/CellCharacterization"> Pseudovibrio denitrificans characteristics </a>
 
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<a href="https://2014.igem.org/Team:Evry/Biology/CellCharacterization"><img src="<img src="https://static.igem.org/mediawiki/2014/c/c8/Mus%C3%A9um.jpg" width="10%"/></a>
 
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<br> Transposons: a transposons
 
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<br><a href="https://2014.igem.org/Team:Evry/Biology/Transposons">To learn more ...</a>
 
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<br> Compounds/target : some usine in the background I think the title need to be change and as to be rename target
 
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<br> <a href="https://2014.igem.org/Team:Evry/Biology/Sensors">To learn more ...</a>
 
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Latest revision as of 00:47, 18 October 2014

IGEM Evry 2014

Overview- Achievements


Medals requirements



Gold Medal

Silver Medal


Bronze Medal

iGEM prizes


Environmental track


  • - Real-time monitoring with a bioactive filtration system, a sponge and its microbiome.

  • - Design with the aim not to introduce a new species in a fragile ecosystem.

  • - Environnementaly safe design, not transferrable to descendance.

  • - Working on an organism people know and are confortable with: the "bath sponge".


Best measurement


  • - Participation to the Interlab Study working not only on the 3 mandatory genetic devices but also the 18 promoters from Anderson’s library.

  • - Designed a phenol biosensor able to detect phenol at lower concentrations than previous teams.


Best Policy & Practices Advance


  • - Extensive report of our philosophical reflexion on the contrast between the ambitions of synthetic biology (rigorous designs) and the reality (trial, error and kludges), which we conducted over the summer.

  • - Debate on the ethical questions raised by the modification of an animal's microbiome, and by the use of an animal as a bioremediation tool in potentially toxic areas.

  • - Reflexion about the risks inherent to the release of genetically modified organisms in seawater, and on the possibility and efficiency of epibiosis as a biological containment.


Best New Basic Part


  • - Bring Transposons Tn10 to the iGEM competition, giving a chance to other teams to develop a project in a wider range of chassis.

Projects Achievements

Chassis characterization


New Vector


  • - Transposon-based vector was created to overcome resistance mechanisms to DNA entrance

  • - Proof of concept to use it in Pseudovibrio denitrificans was achieved


New or Optimized sensors Sensors


Contacts

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