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Team:Evry/Interlab Study/Results
From 2014.igem.org
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The goal of the interlab study is to obtain GFP fluorescence data from the iGEM teams all around the world for about twenty constructions. This will permit to observe the result repeatability across technics, strains and teams. | The goal of the interlab study is to obtain GFP fluorescence data from the iGEM teams all around the world for about twenty constructions. This will permit to observe the result repeatability across technics, strains and teams. | ||
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<a href="https://static.igem.org/mediawiki/parts/0/05/BerkiGEM2006-Promoters.jpg" class="image"> | <a href="https://static.igem.org/mediawiki/parts/0/05/BerkiGEM2006-Promoters.jpg" class="image"> | ||
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<br/>There were two sub-parts in this study: | <br/>There were two sub-parts in this study: | ||
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This library corresponds to 19 constitutive promoters containing some nucleotide mutations on the -35 and the -10, as shown figure x. The idea is to compare the expression strength of promoters according to mutations, from the maximum amount of fluorescence data in order to have significant results. | This library corresponds to 19 constitutive promoters containing some nucleotide mutations on the -35 and the -10, as shown figure x. The idea is to compare the expression strength of promoters according to mutations, from the maximum amount of fluorescence data in order to have significant results. | ||
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<br/> To test the expression of promoters, they are cloned into the PSB1C3 vector followed by the BBa_E0240, a GFP generator. Constructions are inserted in E. coli DH5 alpha that grown on LB medium added with the selection agent, chloramphenicol. Our team decided to test the fluorescence with a | <br/> To test the expression of promoters, they are cloned into the PSB1C3 vector followed by the BBa_E0240, a GFP generator. Constructions are inserted in E. coli DH5 alpha that grown on LB medium added with the selection agent, chloramphenicol. Our team decided to test the fluorescence with a | ||
Revision as of 12:44, 11 October 2014
Interlab study - Aim & Results
The goal of the interlab study is to obtain GFP fluorescence data from the iGEM teams all around the world for about twenty constructions. This will permit to observe the result repeatability across technics, strains and teams.
Anderson collection promoter sequences and mutations.
To test the expression of promoters, they are cloned into the PSB1C3 vector followed by the BBa_E0240, a GFP generator. Constructions are inserted in E. coli DH5 alpha that grown on LB medium added with the selection agent, chloramphenicol. Our team decided to test the fluorescence with a TECAN infiniteM200 in 96 well plates.
To test the expression of promoters, they are cloned into the PSB1C3 vector followed by the BBa_E0240, a GFP generator. Constructions are inserted in E. coli DH5 alpha that grown on LB medium added with the selection agent, chloramphenicol. Our team decided to test the fluorescence with a TECAN infiniteM200 in 96 well plates.
Entire Anderson library of constitutive promoters (J23100-J23119)
Required Devices
