Team:Concordia/Project/Toolkit
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- | <h2>Toolkit</h2> | + | <h2>The Concordia Microalgae Toolkit</h2> |
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- | <p><p> | + | <p>Starting with the goal of making microalgae a viable platform in synthetic biology, we set out to create a toolkit of standardised biological parts for use in these organisms. We chose to work with a diverse group of species that included <em>Chlorella vulgaris</em>, <em>Chlorella kessleri</em>, and <em>Chlamydomonas reinhardtii</em>. These species present a broad range of optimum growth conditions and metabolic profiles. </p> |
+ | <p> | ||
+ | Characterised parts in our toolkit include promoters, terminators, fluorescent proteins, localisation tags, antibiotic markers, and CRISPR/Cas. </p> | ||
+ | <p> | ||
+ | Our promoter library offers plant, fungal, and viral promoters of varying strengths for control of heterologous gene expression in microalgae. Our terminators were compared alongside a variety of these promoters. Multiple localization signals were investigated to enable a user to perform targeted gene expression. We also systematically characterised the effective concentration ranges for antibiotic use in a manner not yet seen for microalgae. </p> | ||
+ | <p> | ||
+ | CRISPR/Cas systems have revolutionized genome engineering efforts by providing a quick and efficient means of inserting and deleting DNA from the host’s genome. Our toolkit set out to demonstrate the ability to perform targeted multi-plex genome engineering of microalgae in a manner accessible to any researcher.</p> | ||
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Latest revision as of 22:37, 17 October 2014
The Concordia Microalgae Toolkit
Starting with the goal of making microalgae a viable platform in synthetic biology, we set out to create a toolkit of standardised biological parts for use in these organisms. We chose to work with a diverse group of species that included Chlorella vulgaris, Chlorella kessleri, and Chlamydomonas reinhardtii. These species present a broad range of optimum growth conditions and metabolic profiles.
Characterised parts in our toolkit include promoters, terminators, fluorescent proteins, localisation tags, antibiotic markers, and CRISPR/Cas.
Our promoter library offers plant, fungal, and viral promoters of varying strengths for control of heterologous gene expression in microalgae. Our terminators were compared alongside a variety of these promoters. Multiple localization signals were investigated to enable a user to perform targeted gene expression. We also systematically characterised the effective concentration ranges for antibiotic use in a manner not yet seen for microalgae.
CRISPR/Cas systems have revolutionized genome engineering efforts by providing a quick and efficient means of inserting and deleting DNA from the host’s genome. Our toolkit set out to demonstrate the ability to perform targeted multi-plex genome engineering of microalgae in a manner accessible to any researcher.
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