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Team:BostonU/Parts
From 2014.igem.org
Parts submitted for bronze medal consideration
| Part Name | Part Type | MoClo Level | Antibiotic Resistance | BBa Name |
|---|---|---|---|---|
| C0040_CI | Repressor Fusion Protein | Level 0 | Chloramphenicol | BBa_K1401600 |
| C0080_CI | Repressor Fusion Protein | Level 0 | Chloramphenicol | BBa_K1401601 |
| E0040m_ID | Reporter Fusion Protein | Level 0 | Chloramphenicol | BBa_K1401602 |
| E0030_ID | Repressor Fusion Protein | Level 0 | Chloramphenicol | BBa_K1401603 |
| pTet+pBAD | Tandem Promoter | Level 0 | Chloramphenicol | BBa_K1401007 |
| pBAD+pTet | Tandem Promoter | Level 0 | Chloramphenicol | BBa_K1401008 |
Test devices for the tandem promoters
| Device Name | Device Description | Component Parts | Destination Vector | MoClo Level | Antibiotic Resistance | BBa Name |
|---|---|---|---|---|---|---|
| pTet-I13453RFP | pInducible | pTet-I13453_AB+BCD2_BC+E1010m_CD+B0015_DE | DVL1_AE | Level 1 | Kanamycin | BBa_K1401009 |
| I13453-pTetRFP | pInducible | I13453-pTet_AB+BCD2_BC+C0040_CD+B0015_DE | DVL1_AE | Level 1 | Kanamycin | BBa_K1401010 |
*Note: The pTet used in this experiment is not Bba_R0040.
Test devices for the fusion proteins
| Device Name | Device Description | Component Parts | Destination Vector | MoClo Level | Antibiotic Resistance | BBa Name |
|---|---|---|---|---|---|---|
| pI13B2Rm_FG | pRepressible | I13453_FB+BCD2_BC+E1010m_CD+B0015_DG | DVL1_FG | Level 1 | Kanamycin | BBa_K1401606 |
| pR10B2C40_EF | pRepressible | R0010_EB+BCD2_BC+C0040_CD+B0015_DF | DVL1_EF | Level 1 | Kanamycin | BBa_K1401607 |
| pR10B2C40Y_EF | pRepressible with Fusion Protein | R0010_EB+BCD2_BC+C0040_CI+E0030_ID+B0015_DF | DVL1_EF | Level 1 | Kanamycin | BBa_K1401608 |
| pR10B2Gm_EF | pRepressible | R0010_EB+BCD2_BC+E0040m_CD+B0015_DF | DVL1_EF | Level 1 | Kanamycin | BBa_K1401609 |
| pR10B2Y_EF | pRepressible | R0010_EB+BCD2_BC+E0030_CD+B0015_DF | DVL1_EF | Level 1 | Kanamycin | BBa_K1401610 |
| pR40B2Rm_FG | pRepressible | R0040_FB+BCD2_BC+E1010m_CD+B0015_DG | DVL1_FG | Level 1 | Kanamycin | BBa_K1401611 |
| pR10B2C80_EF | pRepressible | R0010_EB+BCD2_BC+C0080_CD+B0015_DF | DVL1_EF | Level 1 | Kanamycin | BBa_K1401612 |
| pR10B2C80Y_EF | pRepressible with Fusion Protein | R0010_EB+BCD2_BC+C0080_CI+E0030_ID+B0015_DF | DVL1_EF | Level 1 | Kanamycin | BBa_1401613 |
| pR10B2C80Gm_EF | pRepressible with Fusion Protein | R0010_EB+BCD2_BC+C0080_CI+E0040m_ID+B0015_DF | DVL1_EF | Level 1 | Kanamycin | BBa_K1401614 |
| pR10B2C40Gm_EF | pRepressible with Fusion Protein | R0010_EB+BCD2_BC+C0040_CI+E0040m_ID+B0015_DF | DVL1_EF | Level 1 | Kanamycin | BBa_K1401615 |
| pJ00B2C12m_AE | pConstitutive | J23100_AB+BCD2_BC+C0012m_CD+B0015_DE | DVL1_AE | Level 1 | Kanamycin | BBa_K1401616 |
Test devices for the origins of replication
| Device Name | Device Description | Component Parts | Destination Vector | MoClo Level | Antibiotic Resistance | BBa Name |
|---|---|---|---|---|---|---|
| pJ04B2Gm_p15A | pConstitutive Green | J23104_AB+BCD2_BC+E0040_CD+B0015_DE | DVL1_AE_p15A | Level 1 | Kanamycin | BBa_K1401300 |
| pJ04B2Rm_p15A | pConstitutive Red | J23104_AB+BCD2_BC+E1010_CD+B0015_DE | DVL1_AE_p15A | Level 1 | Kanamycin | BBa_K1401301 |
| COXGR_p15A | Coexpression | J23104_AB+BCD2_BC+E0040_CD+B0015_DE+ J23104_EB+BCD2_BC+E1010_CD+B0015_DF |
DVL2_AF_p15A | Level 2 | Ampicillin | BBa_K1401302 |
| COXRG_p15A | Coexpression | J23104_AB+BCD2_BC+E1010_CD+B0015_DE+ J23104_EB+BCD2_BC+E0040_CD+B0015_DF |
DVL2_AF_p15A | Level 2 | Ampicillin | BBa_K1401303 |
