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Team:BostonU/Training
From 2014.igem.org
| This notebook details the concepts, methods, and protocols learned throughout the iGEM process by all of the BU team members. | |
May |
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Week of May 12 |
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| • Learned about synthetic biology, iGEM, and MoClo. • Became familiar with lab part and BioBrick part nomenclature. • Learned how to use miniEugene from CIDAR lab members. • Developed plan for next two weeks that would guide through training of essential lab protocols - specifically plate streaking, minipreps, MoClo, sequencing, and colony PCR. • Began using miniEugene to design architecture of constructs. • Struck out plates with 15 L0 parts, 6 L1 parts, and 2 L2 parts and grew colonies in liquid cultures overnight. |
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Week of May 19 |
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| • Performed minipreps and quantified the DNA concentration of parts using a Nanodrop spectrophotometer. • Sent the parts for sequencing, all but four of which were correctly verified using BLAST. Parts not correctly verified were re-submitted for sequencing. • Transformed verified parts into E. Coli Bioline cells and performed colony PCR and gel electrophoresis. |
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Week of May 26 | |
| • Analyzed gels and picked colonies. • Performed minipreps on gel-verified strains. • Made glycerol stocks of confirmed parts. • Set up and performed MoClo reactions to make 2 new L1 parts. • Transformed MoClo reactions and grew up over the weekend. |
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June | |
Week of June 2 | |
| • Picked colonies, miniprepped, quantifies, and sent parts for sequence verification. • Set up and performed MoClo reaction for 2 new L1 and 2 new L2 parts. • Transformed reactions and grew up overnight. • Brainstormed to further define project goals. |
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Week of June 9 | |
| • Participated in SB2 (Synthetic Biology Boston) and IWBDA (Internatonal Workshop on Bio-Design Automation) workshops hosted at BU. | |
Week of June 16 | |
| • Set up parts for FACS experiment. • Participated in NEGEM meetup. |
