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From 2014.igem.org

Contents 1 Notebook 1.1 Planning 1.1.1 C - Salicylate Inducible Suppressing System 1.1.2 D - Lemon Scent

Notebook

We constitute our team in November 2013 and we have meeting twice a month until April. Then we met every weeks. The Lab work started at the beginning of June.

Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work. Click here to edit this page

Planning

C - Salicylate Inducible Suppressing System

• Rehydration of BioBricks - 23th July
• Transformation of competent E.coli cells - 24th July
• Bacterial culture - 25th July
• Plasmid DNA Purification - 28th July
• Digestion to check & Electrophoresis - 28th July
• Main Digestion & Electrophoresis - 29th July
• DNA purification gel agarose - 30th July
• Ligation - 30th July
• Transformation in DH5 alpha - 31st July
• Bacterial Culture - 31st July
• Liquid Culture - 1st August
• Plasmid DNA Purification - 4th August
• Digestion to check & Electrophoresis - 4th August
• Final Stock - 4th August
• Liquid Culture - 5th August
• Plasmid DNA Purification - 6th August
• Digestion - 6th August
• Ligation - 6th August

D - Lemon Scent

• PCR Cleanup of BBa_K517003 and BBa_K762100 - 6th August