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From 2014.igem.org

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Contents 1 Intro 2 Aim 3 Methods 4 Results

Intro

The basic proposal for juicyprint is the growth of cellulose by bacteria under the control of light. The 660nm light that the Cph1 protein is sensitive to inhibits the growth. Therefore we designed a system to generate the production of a pellicle sheet in the shape of a projected template of the negative image.

Aim

The aim is to provide a basic proof of concept to test how the light sensitive bacteria respond to the stimulus.

Methods

We were unable to fully test the system with the target system. Therefore we simulated the process with just a liquid target.

660nm light is a bright red colour;

Image courtesy of Beer Color Laboratories; http://www.beercolor.com/color_basics1.htm

Results

A thick 660nm camera filter gets very hot when placed near the projector for a period of time.

Therefore I settled on using a home-made red permanent marker pen to make a 35mm slide suitable for projection. The slide was much thinner and didn't absorb so much light and get hot.