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Team:BostonU/Chimera
From 2014.igem.org
| As synthetic biology continues to expand, researchers are producing a greater variety of novel and innovative genetic circuits. This research revolves around a standard design-build-test cycle that defines the timeline of a project from its conception. The design and assembly of constructs depends on a thorough understanding of their individual components, making thorough part characterization data essential. The fact that there is currently little standardization in DBT workflows and poorly documented standard parts libraries represents an increasingly significant stymying factor to the growth of the field, especially as more laboratories continue to share resources and data. We seek to strengthen the traditional design-build-test cycle fundamental to synthetic biology with a formalized workflow defined by bio-design automation software tools and built upon a thoroughly characterized library of parts. |  |
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We aim to design genetic devices made up of several transcriptional units with the help of Eugene, a design software tool developed by the CIDAR Lab at BU. Eugene is based on SBOL (Synthetic Biology Open Language), an open-source standard for in silico representation of genetic designs. Eugene allows the user to specify necessary constraints for a genetic device (such as number of parts, directionality, and order), and outputs SBOL figures that indicate possible circuit topologies. |
