"
Team:Colombia/Interlab
From 2014.igem.org
Interlab Study
This year Colombia Team-iGEM is participating in Interlab Study! We are glad of share our results and measures. You can find them in the tabs below, you can find the specifications of our equipments and the methodology we used as well.
Methods
Text overviewContents |
Methods
Strains and culture
All transformations were carried out in E. coli TOP10. This bacterium was cultures in LB (suplemented with the appropiate antibiotic for each case) at 37 °C.
Transformations and plasmidic DNA extration
This process was carried out as it is indicated in our link protocols page
Validation
The following gels show respectively the plasmids with the devices and the restriction profiles for each of the tree devices digested with EcoRI and PstI. For restrictions we obtained two bands (the plasmid and the device) with the expected sizes in all cases.
GELES :)
You can check the size of each device with the next table:
| Name | Backbone + device / bp | Device / bp | Linearized backbone / bp |
| BBa_I20260 | 5344 | 919 | 4425 |
| BBa_J23101 + BBa_E0240(B0032-E0040-B0015) | 2981 | 911 | 2070 |
| BBa_J23115 + BBa_E0240 (B0032-E0040-B0015) | 2981 | 911 | 2070 |
Measures
hgkhgukghkFluorescence microscopy
fhfhfh
Fluorometry
dtgdxhedh
Measures
hgkhgukghkFluorescence microscopy
fhfhfh
Fluorometry
dtgdxhedh
hikjik
Figure 1. Scatter Plot of changes in Cortisol Levels (nmol/L) in Males, depending on the hour of the day.
jlkjl
Figure 2. Scatter Plot of changes in Cortisol Levels (nmol/L) in Females, depending on the hour of the day.
Ij;ko;
Figure 3. Scatter Plot of changes in Cortisol Levels (nmol/L) in the Mean between Males and Females, depending on the hour of the day with shaded area of threshold identified.
Measures
hgkhgukghkFluorescence microscopy
fhfhfh
Fluorometry
dtgdxhedh
