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Team:Colombia/Interlab
From 2014.igem.org
Interlab Study
This year Colombia Team-iGEM is participating in Interlab Study! We are glad of share our results and measures. You can find them in the tabs below, you can find the specifications of our equipments and the methodology we used as well.
Methods
Text overviewContents |
Methods
Method1
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Method2
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Validation
The following gels show respectively the plasmids with the devices and the restriction profiles for each of the tree devices digested with EcoRI and PstI. For restrictions we obtained two bands (the plasmid and the device) with the expected sizes in all cases.
GELES :)
You can check the size of each device with the next table:
| Name | Sequence |
| FW pqrr4 | CTT GTC TAG ATT GCA TTT TGC AAA TTT TG |
| RV pqrr4 | GGT CAC TAG TCA ATC TAA TAA ATG CAG ATT |
| FW LuxOD47E | CAA TTC TAG AAA AGA GGA GAA ATA CTA GAT G |
| RV LuxOD47E | ATA TAC TAG TTG TGA CTC TAG TAG AGA |
Measures
hgkhgukghkFluorescence microscopy
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Fluorometry
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Measures
hgkhgukghkFluorescence microscopy
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Fluorometry
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Figure 1. Scatter Plot of changes in Cortisol Levels (nmol/L) in Males, depending on the hour of the day.
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Figure 2. Scatter Plot of changes in Cortisol Levels (nmol/L) in Females, depending on the hour of the day.
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Figure 3. Scatter Plot of changes in Cortisol Levels (nmol/L) in the Mean between Males and Females, depending on the hour of the day with shaded area of threshold identified.
Measures
hgkhgukghkFluorescence microscopy
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Fluorometry
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