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User contributions
From 2014.igem.org
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- 20:10, 17 October 2014 (diff | hist) N Harvard BioDesign/20 June 2014 (Created page with "We made a 2% agarose gel and ran our PCR products on the gel. Lane 1 is a 1 kb ladder and 8 is a 100 bp ladder. 2 and 3 are both the pbBE1a product, while lanes 4-7 are all the h...") (top)
- 20:10, 17 October 2014 (diff | hist) N Harvard BioDesign/19 June 2014 (Created page with "We ran a PCR on the pbBE1a backbone as well as a colony PCR on a Mach1 in order to isolate the hybB promoter. We also miniprepped the chromoproteins and pSB3K3 (pHBD 32-35) Discu...") (top)
- 20:09, 17 October 2014 (diff | hist) N Harvard BioDesign/18 June 2014 (Created page with "Miniprepped pHBD 13, 15, 16, 18 (13 and 18 biological replicates since concentrations from previous day’s miniprep were very low) pHBD20 didn’t grow Transformed chromoprotei...") (top)
- 20:09, 17 October 2014 (diff | hist) N Harvard BioDesign/17 June 2014 (Created page with "Some plates didn’t have individual colonies; just smears. This is likely due to the fact that we added too little antibiotic to our plates. Instead of adding 250 µl of CM, we ...") (top)
- 20:08, 17 October 2014 (diff | hist) N Harvard BioDesign/16 June 2014 (Created page with "We got our sequencing results and aligned them to our plasmid in geneious. 3 out of 4 of the sequencing reactions worked. We plan to sequence the entirety of pHBD1ta or pHBD1tb (...") (top)
- 20:06, 17 October 2014 (diff | hist) N Harvard BioDesign/13 June 2014 (Created page with "Plates from the Gibson assembly (pBbE1a-tet-CsgA) transformations grew many colonies! We picked 2 Turbo and 2 MACH1 colonies and inoculated in 5mL LB+Carb to shake up for mini-p...") (top)
- 20:05, 17 October 2014 (diff | hist) N Harvard BioDesign/12 June 2014 (Created page with "PCR Primers from John→ HBD 1-4 HBD 1 & 2 = fwd and rev for pBbE1a-CsgA backbone HBD 3 & 4 = fwd and rev for Tet promoter Backbone template from Bom → pBbE1a-CsgA @ 65ng/...") (top)
- 20:05, 17 October 2014 (diff | hist) N Harvard BioDesign/11 June 2014 (Created page with "Preparing electrocompetent cells Picked two 5mL LSR10 inoculated cultures and added them each to a 500mL Low Na LB broth flask. Flasks were left shaking at 37C and periodically ...") (top)
- 20:03, 17 October 2014 (diff | hist) N Harvard BioDesign/10 June 2014 (Created page with "Picking colonies Picked colonies from 200uL plated PUC19 transformed cells, 10uL plated pBbe1a (- ctrl) transformed cells, and 10uL pBbe1a CsgA Arc’ed cells. There were many c...") (top)
- 20:02, 17 October 2014 (diff | hist) Team:Harvard BioDesign/Notebook
- 19:49, 17 October 2014 (diff | hist) N Harvard BioDesign/9 June 2014 (Created page with "Making Plates! YESCA + 100ug/mL Carb + 25ug/mL CR + 3ug/mL CBB + 0.5mM IPTG YESCA + 25ug/mL Cm + 25ug/mL CR + 3ug/mL CBB + 0.5mM IPTG Pure LB LB+ 100ug/mL Carb LB + 15ug/mL Cm...") (top)
- 19:48, 17 October 2014 (diff | hist) Team:Harvard BioDesign/Notebook
- 19:47, 17 October 2014 (diff | hist) Team:Harvard BioDesign/Notebook
- 19:47, 17 October 2014 (diff | hist) Team:Harvard BioDesign
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