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From 2014.igem.org

We ran a PCR on the pbBE1a backbone as well as a colony PCR on a Mach1 in order to isolate the hybB promoter. We also miniprepped the chromoproteins and pSB3K3 (pHBD 32-35) Discussed methods to increase rate of color change, because we want a timescale of minutes, not hours. Thought about mimicking a mood ring (liquid crystals, perhaps biological) or a chameleon (localizing pigments to center of cell, then dispersing) Best idea was to use change in pH to alter the color of the chromoproteins. Some chromoproteins (pocilloporins) naturally change color as the pH changes. Worked on our NEGEM 3.1 presentation

Activity Log Checked on plates from yesterday Plates had jojoba bean oil and varying concentrations of surfactant The plates grew with the jojoba bean oil and the tween 20 surfactant Plating different types of oils at varying ratios of oil and LB Trying a 10g/15mL gum arabic solution Worried that our low concentrations won’t be viscous enough to make a paint that will stick to the wall or the E. Coli Sadly, our cells did not grow up in time, and we were not able to complete another assay