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From 2014.igem.org

We made a 2% agarose gel and ran our PCR products on the gel. Lane 1 is a 1 kb ladder and 8 is a 100 bp ladder. 2 and 3 are both the pbBE1a product, while lanes 4-7 are all the hybB promoter (which comes in around 400 bp).

Since it appears that the hybB promoter was isolated, we cut out lanes 4-7 and placed in the 4 C fridge over the weekend. The amplification of the pbBE1a plasmid failed, so we set up another PCR reaction to amplify the backbone, this time with 22.4 ng of plasmid as our template.

We also practiced our NEGEM 3.1 presentation and then went to NEGEM 3.1.