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Subteams

Biosafety

This subteam works on mechanisms to prevent E. coli from surviving outside of the fermenter. This team works on an antibiotics free selection system to improve BioBrick cloning and to reduce antibiotics usage as well as on a kill switch system for the realization of a safe future application.

Construction

The construction team works on the realization of a reverse microbial fuel cell (rMFC). The challenge is the design and construction of the bioreactor. There are many aspects which need to be considered, like the material and the dimension of the electrodes or process control issues.

CO₂ Fixation

This subteam works on the transformation of a heterotrophic organism to an autotroph organism. The aim is to realize the whole Calvin cycle. The main focus is due to the use of a carboxysome for CO₂ fixation.

Homepage and Wiki

The homepage and wiki team publishes new information about the team and the project on the wiki as well as on the German team homepage.

Production of Isobutanol

As a proof of concept one team works on the production of isobutanol. In theory there are many carbon sources for producing the key metabolite pyruvate. Our team wants to use the products of the carbon dioxide fixation to produce pyruvate as a precursor for isobutanol. This isobutanol subproject is based on the work of iGEM Team Formosa 2011 and 2012.

Mediators

A mediator must have the ability to be reducible by electricity at the electrodes and must have good properties for an uptake by the cell. These characteristics are for example the size to pass the outer membrane and the capability for an uptake by a protein based electron system that is encoded by appropriate genes.

Modeling

This subteam models the production of isobutanol and other reactions. We want to compare these results with our experimental data in oder to implement them into our project.

Rathenau - SYNENERGENE

Our iGEM team participates at the SYNENERGENE project. In cooperation with the Athena Institut we work on some future scenarios for our project. This subteam has contact to different firms which could participate at the realization of our project.

Social Media and Design

Our social media and design team is responsible for facebook and twitter where our latest information and pictures are published. Additionally they are designing all our project graphics for presentations and webpages.

Sponsoring

This subteam arranges the contact with different possible sponsors. The aim is to acquire enough financial supporters to pay all team costs for participation, travelling and other emerging costs. All sponsors are mentioned in the category partners.