Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes

From 2014.igem.org

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Kits and Enzymes

Kits

Plasmid purification

PCR Clean-Up

PCR and Gel Clean-Up

PCR core system

Promega GoTaq® PCR Core System I

Protein purification

Amicon Ultra- 15

Enzymes

Digestion enzymes

Enzyme	Producer
EcoRI, EcoRI-HF®	NEB
DpnI	Thermo Scientific
NotI	NEB
PstI, PstI-HF®	NEB
SpeI, SpeI-HF®	NEB
XbaI	NEB
EcoRV	Fermentas
HindIII	Fermentas

Polymerases

Enzyme	Producer
GoTaq DNA Polymerase	Promega
KOD Hotstart DNA-polymerase	Merck Millipore
OneTaq DNA-polymerase	NEB
Pfu DNA-polymerase	Promega
Phusion HF DNA-polymerase	Thermo Scientific

Other enzymes

Enzyme	Producer
Antarctic phosphatase	NEB
T4-DNA-Ligase	NEB
T5 exonuclease	NEB

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