1. Cell lines, DNA synthesis and Plasmids
2. Cryopreservation of bacteria
3.Chemically competent cells preparation
4. Preparation of plasmid DNA on a small scale
5. Rapid transformation using Sorbitol
6.Heat-Shock Transformation
7. .BioBrick Restriction Digest
8. Biobrick ligation
9. LB culture medium + Mercury chloride (ML)
10. Cell growth quantification of mercury resistant Escherichia coli DH5α
11. Quantification of Mercury bioaccumulated by metal binding protein (MBP)
12. Quantification of Green Fluorescent Protein
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