Team:UFAM Brazil/Methods

From 2014.igem.org

Protocols

Cell lines, DNA synthesis and Plasmids

Cryopreservation of bacteria

Chemically competent cells preparation

Preparation of plasmid DNA on a small scale

Rapid transformation using Sorbitol

Heat-Shock Transformation

BioBrick Restriction Digest

Biobrick ligation

LB culture medium + Mercury chloride (ML)

Cell growth quantification of mercury resistant Escherichia coli DH5α

Quantification of Mercury bioaccumulated by metal binding protein (MBP)

Quantification of Green Fluorescent Protein