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Team:BostonU/Workflow
From 2014.igem.org
General Chimera Workflow |
Case Study: BU Priority Encoder |
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Phase I - Build and test basic parts. |
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| This notebook details the process undertaken to replace the high copy pMB1 origin in our existing MoClo Level 1 and Level 2 destination vectors (named DVL1 and DVL2, respectively) with lower copy origins. Namely, the ColE1 (~50 plasmids/cell), p15A (~10 plasmids/cell), and pSC101 (~5 plasmids/cell) origins were selected to replace the high copy origin in DVL1 and DVL2. All protocols used in this notebook are found in our protocols section. |
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