Team:BostonU/Workflow

From 2014.igem.org

Revision as of 17:05, 23 September 2014 by Pachecoa (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Workflow

General Chimera Workflow

Case Study: BU Priority Encoder

Phase I - Build and test basic parts.

This notebook details the process undertaken to replace the high copy pMB1 origin in our existing MoClo Level 1 and Level 2 destination vectors (named DVL1 and DVL2, respectively) with lower copy origins. Namely, the ColE1 (~50 plasmids/cell), p15A (~10 plasmids/cell), and pSC101 (~5 plasmids/cell) origins were selected to replace the high copy origin in DVL1 and DVL2.

All protocols used in this notebook are found in our protocols section.

Our Sponsors