Team:Calgary/Notebook/ProtocolManual/General
From 2014.igem.org
General Protocols
Agarose Gel Electrophoresis
- Add TAE Buffer (100 mL) and Agarose (1g) to a flask [for a 1% gel]
- Cover with plastic wrap, poke hole in top. Then microwave flask until agarose is fully dissolved; avoid boiling
- Take flask to fume hood, and allow to cool to touch. Add SYBR safe (4 μL) to agarose, gently swirl to mix
- Gently pour agarose into assembled gel casting tray, removing any bubbles with a pipette tip
- Allow gel to solidify until translucent. Then transfer to running apparatus filled with TAE buffer
- Load samples containing 3 μL loading dye and ~10-15 μL of DNA
- Run gel at 110V until the dye is ~2/3 of the way down the gel (approx. 40 mins)