Team:Evry/Notebook/Transposons/09.01.14

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Purification of pNK2 with Ori muted

The product of PCR is purified with dialyse during 2h.

Competent cells

To do sorbitol 0,20g/L and glycerol 10%.
Pseudovibrio competent with wash sorbitol and glycerol in M9 medium.

Sep 01

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