Team:Evry/Notebook/Transposons/09.01.14

From 2014.igem.org

Picture

Purification of pNK2 with Ori muted

The product of PCR is purified with dialyse during 2h.


Competent cells

To do sorbitol 0,20g/L and glycerol 10%.
Pseudovibrio competent with wash sorbitol and glycerol in M9 medium.

Sep 01