Team:Evry/Notebook/Transposons/09.01.14
From 2014.igem.org
![Picture](https://static.igem.org/mediawiki/2013/9/90/EvryBiology.png)
Purification of pNK2 with Ori muted
The product of PCR is purified with dialyse during 2h.
Competent cells
To do sorbitol 0,20g/L and glycerol 10%.
Pseudovibrio competent with wash sorbitol and glycerol in M9 medium.