The goal of the interlab study is to obtain GFP fluorescence data from the iGEM teams all around the world for about twenty constructions. This will permit to observe the result repeatability across technics, strains and teams.

There were two sub-parts in this study:

• The mandatory one: obtain fluorescence data for three specific genetic devices expressing GFP and compare them. (donner les constructions) (J23100 to J23119).
• The Extra Credit assignments: we choose to study the entire Anderson library of constitutive prokaryotic promoters. This library corresponds to 19 constitutive promoters containing some nucleotide mutations on the -35 and the -10, as shown figure x. The idea is to compare the expression strength of promoters according to mutations, from the maximum amount of fluorescence data in order to have significant results.

Required Devices

The three required constructions shown various fluorescence intensity at OD 600 nm = 0.5, as shown on bar chart on the right. The same trend was observed between OD 600 nm = 0.1 to 0.8(Data shown on Interlab study notebook). Fluorescence intensity curve profile according to OD 600 nm corresponded to the expected profile for a constitutive promoter (Data shown on Interlab study notebook).

Entire Anderson library of constitutive promoters (J23100-J23119)

Anderson collection promoter sequences and mutations.

Anderson collection promoter sequences and mutations.