Cell lines, DNA synthesis and Plasmids
Cryopreservation of bacteria
Chemically competent cells preparation
Preparation of plasmid DNA on a small scale
Rapid transformation using Sorbitol
Heat-Shock Transformation
BioBrick Restriction Digest
Biobrick ligation
LB culture medium + Mercury chloride (ML)
Cell growth quantification of mercury resistant Escherichia coli DH5α
Quantification of Mercury bioaccumulated by metal binding protein (MBP)
Quantification of Green Fluorescent Protein
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