LabNote

June

June 10, 2014

OKAMOTO
Miniprep

Host	Sample
	Vector	Insert
BL21(DE3)	pGEX6P-2	CuMTSE1
BL21(DE3)	pGEX6P-2	CuMTSE2
BL21(DE3)	pGEX6P-2	CuMTS3
BL21(DE3)	pGEX6P-2	CuMTS62

Restriction Digest

Host	Sample		Enzyme	Buffer
	Vector	Insert
BL21(DE3)PlysS	pGEX6P-2	CuMTSE1	EcoR1	Buffer H
BL21(DE3)PlysS	pGEX6P-2	CuMTSE2	EcoR1	Buffer H
BL21(DE3)PlysS	pGEX6P-2	CuMTS3	EcoR1	Buffer H
BL21(DE3)PlysS	pGEX6P-2	CuMTSE62	EcoR1	Buffer H
BL21(DE3)PlysS	pGEX6P-2	CuMTSE1	Sal1, Not1	Buffer H
BL21(DE3)PlysS	pGEX6P-2	CuMTSE2	Sal1, Not1	Buffer H
BL21(DE3)PlysS	pGEX6P-2	CuMTS3	Sal1, Not1	Buffer H
BL21(DE3)PlysS	pGEX6P-2	CuMTSE62	Sal1, Not1	Buffer H

June 11, 2014

KOBAYASHI
AGE

Lane	Sample/Marker		Enzyme
	Vector	Insert
1	λDNA-HindⅢ
2	pGEX6P-2	CuMTSE1	N.D
3	pGEX6P-2	CuMTSE1	EcoR1
4	pGEX6P-2	CuMTSE1	Sal1,Not1
5	pGEX6P-2	CuMTSE2	N.D
6	pGEX6P-2	CuMTSE2	EcoR1
7	pGEX6P-2	CuMTSE2	Sal1, Not1
8	pGEX6P-2	CuMTS3	N.D
9	pGEX6P-2	CuMTS3	EcoR1
10	pGEX6P-2	CuMTS62	N.D
11	pGEX6P-2	CuMTS62	EcoR1
12	pGEX6P-2	CuMTS62	Sal1,Not1

June 12, 2014

KOBAYASHI
Transformation

Host	Sample
	Vector	Insert
BL21(DE3)pLysS	pGEX6P-2	CuMTSE1
BL21(DE3)pLysS	pGEX6P-2	CuMTSE2
BL21(DE3)pLysS	pGEX6P-2	CuMTS3
BL21(DE3)pLysS	pGEX6P-2	CuMTS62

July

7/25

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August

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October

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