Team:Calgary/Project/BsDetector/DevicePrototype

From 2014.igem.org

Revision as of 03:57, 8 September 2014 by Mariyashtil (Talk | contribs)

Device Prototype

Our team decided that in order to ensure that this system is physically feasible that we would aim to produce a working physical prototype. In this process it was imperative that the end-user was considered and that the materials used made our system economically feasible and compatible with the biological system. Through collaboration with the transformers, detectors and our human practices subteams we were able to manufacture a working prototype as the first stage of development.

Process

//Integrate creation paragraph below into this section// Our device is portable and user friendly. A drop of blood from a patient first needs to go through sample preparation to extract target DNA out of the sample. A sample with extracted DNA then flows into the Polymerase Chain Reaction (PCR) chamber where target DNA is amplified via isothermal PCR. Reaction takes a couple of hours to complete and the sample is manually pushed out of the PCR chamber into separate chambers with B. subtilis. In each of the chambers, B. subtilis is engineered to detect a specific disease allowing for multidiagnosis.