Team:Evry/Notebook/CellCharacterization

From 2014.igem.org

(Difference between revisions)
Line 1: Line 1:
 +
{{:Team:Evry/Template:HomeIncludes}}
 +
{{:Team:Evry/Template:Menu}}
 +
{{:Team:Evry/Template:NotebookTop}}
 +
<html>
<html>
-
<head>
+
<script src="http://codyhouse.co/demo/vertical-timeline/js/modernizr.js"></script>
-
</head>
+
<script src="https://2014.igem.org/Team:Evry/JS/Jqueryui?action=raw&ctype=text/javascript"></script>
-
<body>
+
<script src="https://2014.igem.org/Team:Evry/JS/timeline?action=raw&ctype=text/javascript"></script>
 +
<link rel="stylesheet" href="https://2014.igem.org/Team:Evry/CSS/timeline?action=raw&ctype=text/css" type="text/css" />
 +
</html>
-
<div class="week">
+
{{:Team:Evry/Notebook/CellCharacterization/Antibiotic_test}}
-
  <h5 class=title2> </h5>
+
-
  <div class="CellCharacterization">
+
-
<p>
 
-
<a href="https://2014.igem.org/Team:Evry/Notebook/CellCharacterization/Antibiotic_test">Antibiotic test</a>
 
-
 
+
<html>
-
 
+
</div>
-
</p>
+
</section>
-
 
+
-
  </div>
+
-
  </div>
+
-
</body>
+
</html>
</html>
 +
 +
{{:Team:Evry/Template:HomeFooter}}

Revision as of 16:51, 24 August 2014

IGEM Evry 2014

Notebook

Preparation of antibiotic stocks

Antibiotic Stock concentration Protocol
Kanamycin 25 mg/mL Weight 0.56g of Kanamycin powder, solubilization into 20mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)

Streptomycin 100 mg/mL Weight 1g of Streptomycin powder, solubilization into 20mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)

Amplicilin 100 mg/mL Weight 1g of Amplicilin powder, solubilization into 20mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)

Tetracyclin 15 mg/mL Weight 0.6g of Tetracyclin powder, solubilization into 20mL of ethanol 50%.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C and hiden from light(aliquots of 1mL)
Chloramphenicol 34 mg/mL Weight 0.34g of Chloramphenicol powder, solubilization into 10mL of ethanol 100%.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C and hiden from light(aliquots of 1mL)


Contacts

Retrieved from "http://2014.igem.org/Team:Evry/Notebook/CellCharacterization"