Team:Glasgow/Modeling Part2 align=

From 2014.igem.org

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The following graphs show the effect of gas vesicles on the upwards speed of E. Coli, over 24 hours.
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The following giff shows the effect of gas vesicles on the upwards speed of E. Coli, over 24 hours.
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<p>The gif also compares salt water to fresh.  Saltwater has a higher density than fresh, and so the bacteria <br>experience a greater buoyancy force. As we can see, they don't move very fast!  This is to be expected, as they <br>exist on a much smaller scale than us, who are used to seeing things moving in meters per second.<br><br>
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It's generally well known that, for positive buoyancy to occur, the maximum space the gas vesicles can fill is 10% - <br>any more than this, and they begin to have a negative effect on the cell's protein resources.  We see that, in <br>our model, upwards movement was attained at ~12% filling.  This is a bit larger than normal, but again could be <br>due to the simplification of the model and density calculations – more of the bacterial mass could have been <br>replaced than we are estimating.
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<br><br>The main problem we see is our speed – it really is very slow!  Based on this data, it would take a 50% <br>filled bacteria 24 hours to travel 2cm – a speed of 0.25um a second. This is in the very low end of the <br>literature estimate, which is quoted at <2um/s, and is at a much higher % filling.  We are at least confident <br>that we're not out by a large amount.

Revision as of 09:59, 8 August 2014

Bubble Test Page








Section 2: Bacteria and Buoyancy With the main focus of our project being the gas vesicles, we decided to model the effect that they would have on the
bacterium's path.

The net force on the bacterium should be positive for floatation to occur. This force is composed of three components:
The buoyancy force, the force due to gravity and Stokes drag force. They are applied thus:


Normally, a bacterium's density is greater than water (E.Coli is about 1100kg/m^3, compared to water's 998kg/m^3
at 20 degrees Celcius). The presence of gas vesicles replaces some of the mass of the cell with lighter gas,
thus reducing the density.
As with the random walk model, we'll state our assumptions up here.

  • The density of a gas vacoule would be 250kg/m3, a value obtained by Walsby in his study of the cyanobacteria anabaea flos-aquae
  • It is believed that the bacteria will increase in volume as a result of gas vesicle production. We'll roughly estimate this to be half the % of total volume the vesicles will fill.
  • Again, we assume the bacteria is a sphere.

With some preliminary calculations, we decided that the movement would be essentially linear – while there is an acceleration phase at the beginning while the forces are unbalanced, the time it takes to reach a constant terminal velocity is negligible compared to the time for which we're observing the system.

One of the problems with this model is finding the E. Coli mass that will be replaced by the vesicles – we need this in order to find the final density. This was estimated by replacing a given volume (5, 10, 15 etc), with an equivalent (lower density) volume of gas vesicle.

We would use the density and the assumptions above to calculate a new density for the bacteria. This is put into the terminal velocity equation:
The following giff shows the effect of gas vesicles on the upwards speed of E. Coli, over 24 hours.

The gif also compares salt water to fresh. Saltwater has a higher density than fresh, and so the bacteria
experience a greater buoyancy force. As we can see, they don't move very fast! This is to be expected, as they
exist on a much smaller scale than us, who are used to seeing things moving in meters per second.

It's generally well known that, for positive buoyancy to occur, the maximum space the gas vesicles can fill is 10% -
any more than this, and they begin to have a negative effect on the cell's protein resources. We see that, in
our model, upwards movement was attained at ~12% filling. This is a bit larger than normal, but again could be
due to the simplification of the model and density calculations – more of the bacterial mass could have been
replaced than we are estimating.

The main problem we see is our speed – it really is very slow! Based on this data, it would take a 50%
filled bacteria 24 hours to travel 2cm – a speed of 0.25um a second. This is in the very low end of the
literature estimate, which is quoted at <2um/s, and is at a much higher % filling. We are at least confident
that we're not out by a large amount. Click here to edit this page