Team:Glasgow/Parts

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Biobricks

The following table lists the biobricks we created. Many of these have additional characterisation information, available on their respective Registry pages.
We have also put together a Motility Parts Collection
Some important parts:

  • BBa_B0032 - Weak Ribosome Binding Site
  • BBa_B0034 - Strong Ribosome Binding Site
  • BBa_J61002 - Plasmid Vector
  • BBa_J23xxx - Promoter

Please note that all parts highlighted in RED have been submitted and received by the registry.

Biobrick Number Type Description Designer Length (BP) Design Notes
BBa_K1463000 Composite Switch Biobrick with RFP and GFP. BBa_K1463520, BBa_K1463560, BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0034, BBa_E0040 iGEM14_Glasgow 1744
BBa_K1463001 Composite Switch Biobrick with just GFP. BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0034, BBa_E0040 iGEM14_Glasgow 1013
BBa_K1463002 Composite Switch Biobrick with just GFP weaker RBS. BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0032, BBa_E0040 iGEM14_Glasgow 1014
BBa_K1463030 Basic Switch nucleotide spacer iGEM14_Glasgow 11
BBa_K1463040 Basic φC31 attP site iGEM14_Glasgow 54
BBa_K1463041 Basic φC31 attB site iGEM14_Glasgow 55
BBa_K1463050 Composite K1463040, K1463030, K1463501, B0010, K1463041 iGEM14_Glasgow 267
BBa_K1463100 GvpA RBS iGEM14_Glasgow 8
BBa_K1463205 Composite B0034, K737016 iGEM14_Glasgow 237
BBa_K1463207 Composite J61002, J23100, B0034, K737016 iGEM14_Glasgow 3226
BBa_K1463210 Composite K1463100, K737016 iGEM14_Glasgow 233
BBa_K1463225 Composite GvpC(K737017) and B0032 iGEM14_Glasgow 523
BBa_K1463340 Composite B0034, K737016, B0032, K737017 iGEM14_Glasgow 764
BBa_K1463345 Composite J61002, J23100, B0034, K737016, B0032, K737017 iGEM14_Glasgow 3767
BBa_K1463350 Composite K1463100, K737016, B0032, K737017 iGEM14_Glasgow 764
BBa_K1463400 φC31 Gp3 iGEM14_Glasgow 738
BBa_K1463401 Composite B0034, K1463400 iGEM14_Glasgow 756
BBa_K1463501 Promoter J23100 promoter in reverse iGEM14_Glasgow 38
BBa_K1463520 Basic E1010 RFP in reverse iGEM14_Glasgow 705
BBa_K1463560 Basic Strong reverse RBS iGEM14_Glasgow 10
BBa_K1463600 Basic FliC iGEM14_Glasgow 1497
BBa_K1463601 Composite B0034, K1463600 iGEM14_Glasgow 1515
BBa_K1463602 Composite J23116, B0032, B0034, K1463600 iGEM14_Glasgow 1579
BBa_K1463603 Composite J23106, B0032, B0034, K1463600 iGEM14_Glasgow 1579
BBa_K1463604 Composite J23100, B0032, B0034, K1463600 iGEM14_Glasgow 1579
BBa_K1463700 Basic MotA 888
BBa_K1463701 Composite B0032, K1463700 iGEM14_Glasgow 907
BBa_K1463702 Composite J23100, B0032, K1463700 iGEM14_Glasgow 950
BBa_K1463703 Composite J23103, B0032, K1463700 iGEM14_Glasgow 950
BBa_K1463704 Composite J23106, B0032, K1463700 iGEM14_Glasgow 950
BBa_K1463705 Composite J23112, B0032, K1463700 iGEM14_Glasgow 950
BBa_K1463706 Composite J23116, B0032, K1463700 iGEM14_Glasgow 950
BBa_K1463750 Basic MotB iGEM14_Glasgow 927
BBa_K1463751 Composite B0032, K1463750 iGEM14_Glasgow 946
BBa_K1463752 Composite J23100, B0032, K1463750 iGEM14_Glasgow 989
BBa_K1463753 Composite J23103, B0032, K1463750 iGEM14_Glasgow 989
BBa_K1463754 Composite J23106, B0032, K1463750 iGEM14_Glasgow 989
BBa_K1463755 Composite J23112, B0032, K1463750 iGEM14_Glasgow 989
BBa_K1463756 Composite J23116, B0032, K1463750 iGEM14_Glasgow 989
BBa_K1463770 Composite J23103, B0032, K1463700, B0032, K1463750 iGEM14_Glasgow 1904
BBa_K1463771 Composite J23112, B0032, K1463700, B0032, K1463750 iGEM14_Glasgow 1904
BBa_K1463772 Composite J23106, B0032, K1463700, B0032, K1463750 iGEM14_Glasgow 1904
BBa_K1463773 Composite J23116, B0032, K1463700, B0032, K1463750 iGEM14_Glasgow 1904
BBa_K1463805 Composite J61002, J23103, E1010 iGEM14_Glasgow 3703
BBa_K1463806 Composite J61002, J23106, E1010 iGEM14_Glasgow 3703
BBa_K1463807 Composite J61002, J23112, E1010 iGEM14_Glasgow 3703
BBa_K1463808 Composite J61002, J23116, E1010 iGEM14_Glasgow 3703

Motility Parts Collection

The motility parts collection is the culmination of our work on bacterial motility genes - MotA, MotB and FliC. Our project plan required that, for the bacteria to float, these genes would need switched off. Therefore, work had to be done to:

  • Knock out the genes from the bacteria
  • Be able to recover the motility
  • Make the parts suitable for placement on one side of the recombinase switch
Contain MotB Contain MotA Contain FliC
Part Number Description Part Number Description Part Number Description
BBa_K1463750 Basic MotB part BBa_K1463700 Basic MotA BBa_K1463600 The basic FliC part
BBa_K1463751 Composite B0032, K1463750 BBa_K1463701 Composite B0032, K1463700 BBa_K1463601 Composite B0034, K1463600
BBa_K1463752 Composite J23100, B0032, K1463750 BBa_K1463702 Composite J23100, B0032, K1463700 BBa_K1463602 Composite J23116, B0032, B0034, K1463600
BBa_K1463753 Composite J23103, B0032, K1463750 BBa_K1463703 Composite J23103, B0032, K1463700 BBa_K1463603 Composite J23106, B0032, B0034, K1463600
BBa_K1463754 Composite J23106, B0032, K1463750 BBa_K1463704 Composite J23106, B0032, K1463700 BBa_K1463604 Composite J23100, B0032, B0034, K1463600
BBa_K1463755 Composite J23112, B0032, K1463750 BBa_K1463705 Composite J23112, B0032, K1463700
BBa_K1463756 Composite J23116, B0032, K1463750 BBa_K1463706 Composite J23116, B0032, K1463700
Contain MotA and MotB
Part Number Description
BBa_K1463770 Composite J23103, B0032, K1463700, B0032, K1463750
BBa_K1463771 Composite J23112, B0032, K1463700, B0032, K1463750
BBa_K1463772 Composite J23106, B0032, K1463700, B0032, K1463750
BBa_K1463773 Composite J23116, B0032, K1463700, B0032, K1463750
BBa_K1463760 MotA and MotB with B0032 RBS
BBa_K1463774 MotA and MotB under J23100 Promoter with B0032 RBS.
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