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Team:Paris Saclay/Project
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===A - The chassis coli Odor free=== | ===A - The chassis coli Odor free=== | ||
Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene. | Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene. | ||
| - | In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but | + | [[File:IndoleFormation.jpg|500px|center]] |
| + | In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but this strain was too modified to be used for our project. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all. | ||
[[File:OdorFree.jpg|500px|center]] | [[File:OdorFree.jpg|500px|center]] | ||
Revision as of 07:47, 6 August 2014
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Contents |
The different parts of our project:
A - The chassis coli Odor free
Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene.
In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but this strain was too modified to be used for our project. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all.
