Team:Paris Saclay/Protocols

From 2014.igem.org

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#[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_clean-up PCR clean-up]
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_clean-up PCR clean-up]
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_for_bacterial_culture PCR for bacterial culture]
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_for_bacterial_culture PCR for bacterial culture]
 +
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_competent_cells_by_electroporation Transformation of competent cells by electroporation]

Revision as of 20:58, 4 August 2014

Protocols

  1. Preparation of supercompetent E.coli cells
  2. Transformations of supercompetent E. coli cells
  3. Extraction of the Genomic DNA from Bacteria by using NucleoSpin® Tissue
  4. Extraction of the Genomic DNA from Bacteria without NucleoSpin® Tissue
  5. Gel Electrophoresis
  6. Transformation of CaCl2 supercompetent E.coli cells
  7. PCR for the genomic DNA of bacteria
  8. PCR clean-up
  9. PCR for bacterial culture
  10. Transformation of competent cells by electroporation
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