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Team:Paris Saclay/Project
From 2014.igem.org
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<tr><td bgColor="#e7e7e7" colspan="3" height="1px">Page under construction. Our team is working hard and excited to develop a very good Wiki.</tr> | <tr><td bgColor="#e7e7e7" colspan="3" height="1px">Page under construction. Our team is working hard and excited to develop a very good Wiki.</tr> | ||
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| - | + | ==The different parts of our project:== | |
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| + | ===A - The chassis coli Odor free=== | ||
Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene. | Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene. | ||
| - | In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but it wasn't the right strain. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all. | + | In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but it wasn't the right strain. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all. |
| - | + | [[File:OdorFree.jpg|500px|center]] | |
| - | + | ===B - Construction of the fusion protein (color)=== | |
| - | + | ===C - Salicylate Inducible Suppressing System=== | |
| - | + | ===D - Lemon scent=== | |
| - | + | ===E - Banana scent=== | |
| - | + | ===F - The lemon shaping=== | |
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Revision as of 16:02, 4 August 2014
| Page under construction. Our team is working hard and excited to develop a very good Wiki. | ||
Contents |
The different parts of our project:
A - The chassis coli Odor free
Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene. In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but it wasn't the right strain. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all.
