Team:Waterloo/Deliver

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       <h2>Overview: Delivering Antibiotic Susceptibility in vivo</h2>
       <h2>Overview: Delivering Antibiotic Susceptibility in vivo</h2>
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<p>We decided to use conjugation as our mode of delivery because it has a large carrying capacity. Our silencing systems are quite large and therefore need the appropriate delivery mechanism. While a Staphylococcus virus would be more efficient at transferring DNA to a recipient cell, there are no Staphylococcus viruses that have been found with a carrying capacity large enough to handle our silencing systems.
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Conjugation is a transfer of genetic material between prokaryotes by cell-to-cell contact. This process is well characterized in gram-negative bacteria, such as E. coli, but it is less characterized in gram-positive bacteria like Staphylococcus aureus. To infer information on the gram-positive process, microbiologists usually use the gram-negative process as a template (Grohmann et al, 2003).
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The conjugation process in both gram-positive and gram-negative bacteria all share underlying similarities. For example, an origin of transfer (oriT) is required for all plasmid conjugation. A multiprotein complex that binds to the origin of transfer is called the relaxosome. The proteins involved in the relaxosome are coded within a tra/trs region that can be found on the plasmid or on the chromosome. The relaxosome is recognized by DNA relaxes which are able to perform a single or double stranded cut (depending on the type of system). The DNA released from the cut is then available to transfer to the recipient cells (Grohmann et al, 2003).
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Revision as of 01:39, 18 October 2014

Deliver

Overview: Delivering Antibiotic Susceptibility in vivo

We decided to use conjugation as our mode of delivery because it has a large carrying capacity. Our silencing systems are quite large and therefore need the appropriate delivery mechanism. While a Staphylococcus virus would be more efficient at transferring DNA to a recipient cell, there are no Staphylococcus viruses that have been found with a carrying capacity large enough to handle our silencing systems. Conjugation is a transfer of genetic material between prokaryotes by cell-to-cell contact. This process is well characterized in gram-negative bacteria, such as E. coli, but it is less characterized in gram-positive bacteria like Staphylococcus aureus. To infer information on the gram-positive process, microbiologists usually use the gram-negative process as a template (Grohmann et al, 2003). The conjugation process in both gram-positive and gram-negative bacteria all share underlying similarities. For example, an origin of transfer (oriT) is required for all plasmid conjugation. A multiprotein complex that binds to the origin of transfer is called the relaxosome. The proteins involved in the relaxosome are coded within a tra/trs region that can be found on the plasmid or on the chromosome. The relaxosome is recognized by DNA relaxes which are able to perform a single or double stranded cut (depending on the type of system). The DNA released from the cut is then available to transfer to the recipient cells (Grohmann et al, 2003).

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