Team:Glasgow/Parts

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Revision as of 23:56, 17 October 2014

Bubble Test Page

Biobricks

The following table lists the biobricks we created. Many of these have additional characterisation information, available on their respective Registry pages.
We have also put together a Motility Parts Collection
Some important parts:

BBa_B0032 - Weak Ribosome Binding Site
BBa_B0034 - Strong Ribosome Binding Site
BBa_J61002 - Plasmid Vector
BBa_J23xxx - Promoter

Please note that all parts highlighted in RED have been submitted and received by the registry.

Biobrick Number	Type	Description	Designer	Length (BP)
BBa_K1463000	Composite	Switch Biobrick with RFP and GFP. BBa_K1463520, BBa_K1463560, BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0034, BBa_E0040	iGEM14_Glasgow	1744
BBa_K1463001	Composite	Switch Biobrick with just GFP. BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0034, BBa_E0040	iGEM14_Glasgow	1013
BBa_K1463002	Composite	Switch Biobrick with just GFP weaker RBS. BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0032, BBa_E0040	iGEM14_Glasgow	1014
BBa_K1463030	Basic	Switch nucleotide spacer	iGEM14_Glasgow	11
BBa_K1463040	Basic	φC31 attP site	iGEM14_Glasgow	54
BBa_K1463041	Basic	φC31 attB site	iGEM14_Glasgow	55
BBa_K1463050	Composite	K1463040, K1463030, K1463501, B0010, K1463041	iGEM14_Glasgow	267
BBa_K1463100		GvpA RBS	iGEM14_Glasgow	8
BBa_K1463205	Composite	B0034, K737016	iGEM14_Glasgow	237
BBa_K1463207	Composite	J61002, J23100, B0034, K737016	iGEM14_Glasgow	3226
BBa_K1463210	Composite	K1463100, K737016	iGEM14_Glasgow	233
BBa_K1463225	Composite	GvpC(K737017) and B0032	iGEM14_Glasgow	523
BBa_K1463340	Composite	B0034, K737016, B0032, K737017	iGEM14_Glasgow	764
BBa_K1463345	Composite	J61002, J23100, B0034, K737016, B0032, K737017	iGEM14_Glasgow	3767
BBa_K1463350	Composite	K1463100, K737016, B0032, K737017	iGEM14_Glasgow	764
BBa_K1463400		φC31 Gp3	iGEM14_Glasgow	738
BBa_K1463401	Composite	B0034, K1463400	iGEM14_Glasgow	756
BBa_K1463501	Promoter	J23100 promoter in reverse	iGEM14_Glasgow	38
BBa_K1463520	Basic	E1010 RFP in reverse	iGEM14_Glasgow	705
BBa_K1463560	Basic	Strong reverse RBS	iGEM14_Glasgow	10
BBa_K1463600	Basic	FliC	iGEM14_Glasgow	1497
BBa_K1463601	Composite	B0034, K1463600	iGEM14_Glasgow	1515
BBa_K1463602	Composite	J23116, B0032, B0034, K1463600	iGEM14_Glasgow	1579
BBa_K1463603	Composite	J23106, B0032, B0034, K1463600	iGEM14_Glasgow	1579
BBa_K1463604	Composite	J23100, B0032, B0034, K1463600	iGEM14_Glasgow	1579
BBa_K1463700	Basic	MotA		888
BBa_K1463701	Composite	B0032, K1463700	iGEM14_Glasgow	907
BBa_K1463702	Composite	J23100, B0032, K1463700	iGEM14_Glasgow	950
BBa_K1463703	Composite	J23103, B0032, K1463700	iGEM14_Glasgow	950
BBa_K1463704	Composite	J23106, B0032, K1463700	iGEM14_Glasgow	950
BBa_K1463705	Composite	J23112, B0032, K1463700	iGEM14_Glasgow	950
BBa_K1463706	Composite	J23116, B0032, K1463700	iGEM14_Glasgow	950
BBa_K1463750	Basic	MotB	iGEM14_Glasgow	927
BBa_K1463751	Composite	B0032, K1463750	iGEM14_Glasgow	946
BBa_K1463752	Composite	J23100, B0032, K1463750	iGEM14_Glasgow	989
BBa_K1463753	Composite	J23103, B0032, K1463750	iGEM14_Glasgow	989
BBa_K1463754	Composite	J23106, B0032, K1463750	iGEM14_Glasgow	989
BBa_K1463755	Composite	J23112, B0032, K1463750	iGEM14_Glasgow	989
BBa_K1463756	Composite	J23116, B0032, K1463750	iGEM14_Glasgow	989
BBa_K1463770	Composite	J23103, B0032, K1463700, B0032, K1463750	iGEM14_Glasgow	1904
BBa_K1463771	Composite	J23112, B0032, K1463700, B0032, K1463750	iGEM14_Glasgow	1904
BBa_K1463772	Composite	J23106, B0032, K1463700, B0032, K1463750	iGEM14_Glasgow	1904
BBa_K1463773	Composite	J23116, B0032, K1463700, B0032, K1463750	iGEM14_Glasgow	1904
BBa_K1463805	Composite	J61002, J23103, E1010	iGEM14_Glasgow	3703
BBa_K1463806	Composite	J61002, J23106, E1010	iGEM14_Glasgow	3703
BBa_K1463807	Composite	J61002, J23112, E1010	iGEM14_Glasgow	3703
BBa_K1463808	Composite	J61002, J23116, E1010	iGEM14_Glasgow	3703

Motility Parts Collection

The motility parts collection is the culmination of our work on bacterial motility genes - MotA, MotB and FliC. Our project plan required that, for the bacteria to float, these genes would need switched off. Therefore, work had to be done to:

Knock out the genes from the bacteria
Be able to recover the motility
Make the parts suitable for placement on one side of the recombinase switch

Contain MotB		Contain MotA		Contain FliC
Part Number	Description	Part Number	Description	Part Number	Description
BBa_K1463750	Basic MotB part	BBa_K1463700	Basic MotA	BBa_K1463600	The basic FliC part
BBa_K1463751	Composite B0032, K1463750	BBa_K1463701	Composite B0032, K1463700	BBa_K1463601	Composite B0034, K1463600
BBa_K1463752	Composite J23100, B0032, K1463750	BBa_K1463702	Composite J23100, B0032, K1463700	BBa_K1463602	Composite J23116, B0032, B0034, K1463600
BBa_K1463753	Composite J23103, B0032, K1463750	BBa_K1463703	Composite J23103, B0032, K1463700	BBa_K1463603	Composite J23106, B0032, B0034, K1463600
BBa_K1463754	Composite J23106, B0032, K1463750	BBa_K1463704	Composite J23106, B0032, K1463700	BBa_K1463604	Composite J23100, B0032, B0034, K1463600
BBa_K1463755	Composite J23112, B0032, K1463750	BBa_K1463705	Composite J23112, B0032, K1463700
BBa_K1463756	Composite J23116, B0032, K1463750	BBa_K1463706	Composite J23116, B0032, K1463700
Contain MotA and MotB
Part Number		Description
BBa_K1463770		Composite J23103, B0032, K1463700, B0032, K1463750
BBa_K1463771		Composite J23112, B0032, K1463700, B0032, K1463750
BBa_K1463772		Composite J23106, B0032, K1463700, B0032, K1463750
BBa_K1463773		Composite J23116, B0032, K1463700, B0032, K1463750
BBa_K1463760		MotA and MotB with B0032 RBS
BBa_K1463774		MotA and MotB under J23100 Promoter with B0032 RBS.

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