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Team:Evry/Biology/Transposons
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| + | <h1> Transposas Tn10 / Is10 </h1> | ||
| + | <div align="right">Here the text</div>The complex Tn10/IS10 is involved in the non-replicative cut-and-paste mechanism.; the transposable segment is excised by cleavage at the transposon ends and then re-inserted in a target DNA site. | ||
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| + | Tn10 transposase protein is made up of 402 amino-acid, which recognise inverted repeats insertion sequence; Is10-right and Is10-left. | ||
| + | Tn10 is a composite bacterial transposon subject to a strong positive and negative regulation. | ||
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| + | <div align="right">Here the text</div>IS 10 is an insertion sequence composing the transposon Tn10. The two Is10 elements, Is10-Right and Is10-Left, contain all of the Tn10 encoded genetic determinants; as the coding region for the transposase protein Tn10. The two ends of IS-10 have a similar terminal inverted repeat of 23bp, correspond to the transposase-binding site. However some genetic drift between both result in different functionality; Is10-right is fully functional while Is10-left is partially functional. | ||
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Revision as of 19:41, 17 October 2014
Biology - Transposons
Transposon
Here the text
A transposon is a sequence of DNA able to move by itself within the genome preferentially in genic regions; characterized as mobile genetic element or transposable element. This event
occur according differents transposition mechanism.
Here the text
One of them called cut-and-paste mechanism require an enzyme : transposase. This enzyme is encoded within some transposons. Transposase binds to the end of the transposon, which is consist of
inverted repeats and catalyze the movement of transposon for insertion and excision.
Transposas Tn10 / Is10
Here the text
The complex Tn10/IS10 is involved in the non-replicative cut-and-paste mechanism.; the transposable segment is excised by cleavage at the transposon ends and then re-inserted in a target DNA site.
Tn10 transposase protein is made up of 402 amino-acid, which recognise inverted repeats insertion sequence; Is10-right and Is10-left. Tn10 is a composite bacterial transposon subject to a strong positive and negative regulation.
Here the text
IS 10 is an insertion sequence composing the transposon Tn10. The two Is10 elements, Is10-Right and Is10-Left, contain all of the Tn10 encoded genetic determinants; as the coding region for the transposase protein Tn10. The two ends of IS-10 have a similar terminal inverted repeat of 23bp, correspond to the transposase-binding site. However some genetic drift between both result in different functionality; Is10-right is fully functional while Is10-left is partially functional.
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