Team:UTK-Knoxville/Parts

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:UTK-Knoxville/Parts&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<p id="title">Design Construction and Characterization<br>of Modular Chimeric Biosensors</p>
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<h2 align="center">Gene Descriptions</h2>
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<hr><h3 id="top">OmpR and p-OmpR</h3><hr>
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<p>&nbsp;&nbsp;&nbsp;OmpR is a transcriptional regulator native to E. coli. It is part of the
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EnvZ/OmpR two-component signal transduction system and is controlled by the kinase EnvZ. In wildtype
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E. coli, EnvZ upregulates OmpR in high osomlarity conditions, phosphorylating it to create p-OmpR. </p><br><br>
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<hr><h3>OmpC and OmpF</h3><hr>
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<p>&nbsp;&nbsp;&nbsp;OmpC is an E. coli Promoter that is activated by the EnvZ/OmpR signal transduction system.
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In the presence of phosphorylated OmpR (called p-OmpR), transcription of OmpC is allowed and genes under the control
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of OmpC are expressed. In wildtype E. coli, when no p-OmpR is present within the cell, OmpR instead regulates the
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expression of the promoter OmpF.</p><br><br>
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<hr><h3>EnvZ</h3><hr>
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<p>&nbsp;&nbsp;&nbsp;The sensory histidine kinase EnvZ is responsible for controlling the function of OmpR,
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which in turn upregulates either OmpC or OmpF. EnvZ undergoes autophosphorylation. In high osmolarity conditions,
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this phosphate group is passed to OmpR, generating p-OmpR. The mechanism by which EnvZ responds to osmolarity is
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unknown. EnvZ is located in the inner membrane of the cell, containing two transmembrane domains, a cytoplasmic
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domain, and a periplasmic domain.</p><br><br>
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<!--Parts Submitted to the Registry  -->
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<hr><h3>RFP</h3><hr>
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<tr><td > <h3> Parts Submitted to the Registry </h3></td>
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<p>&nbsp;&nbsp;&nbsp;The RFP we use comes from the iGEM registry (<a href="<a href="http://parts.igem.org/Part:BBa_E1010">
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<td ></td >
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  part BBa_E1010</a>). It is native to the coral
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<td > <h3>What information do I need to start putting my parts on the Registry? </h3></td>
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Discosoma striata. The exctitation wavelength of RFP is 584nm and it has an emission wavelength of 607nm.</p><br>
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will submit them to the <a href="http://partsregistry.org"> Registry of Standard Biological Parts</a>. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.
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<p>
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<hr><h3>Tar</h3><hr>
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<strong>Note that if you want to document a part you need to document it on the <a href="http://partsregistry.org Registry"> Registry</a>, not on your team wiki.</strong> Future teams and other users and are much more likely to find parts on the Registry than on your team wiki.  
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<p>Tar is an MCP (methyl-accepting chemotaxis protein) native to E. coli
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</p>
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that responds primarily to aspartate. Tar contains two transmembrane domains,
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and a periplasmic ligand sensing domain, which recognizes the presence of aspartate via binding to this region. </p>
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<br><br><br>
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<p>
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<img id="UTK"alt="UTK" src="https://static.igem.org/mediawiki/2014/8/88/UTK.png" />
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Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
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<!-- end of page -->
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<h3>When should you put parts into the Registry?</h3>
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</body>
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<p>
 
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As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements.
 
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The information needed to initially create a part on the Registry is:
 
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part <a href="http://parts.igem.org/Part:BBa_K404003">BBa_K404003</a> for an excellent example of a highly characterized part.
 
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You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry"> Add a Part to the Registry</a> link.
 
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<tr><td colspan="3" > <h3> Parts Table</h3></td></tr>
 
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Any parts your team has created will appear in this table below:</td></tr>
 
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<groupparts>iGEM013 UTK-Knoxville</groupparts>
 

Revision as of 20:17, 15 July 2014

Gene Descriptions

OmpR and p-OmpR

   OmpR is a transcriptional regulator native to E. coli. It is part of the EnvZ/OmpR two-component signal transduction system and is controlled by the kinase EnvZ. In wildtype E. coli, EnvZ upregulates OmpR in high osomlarity conditions, phosphorylating it to create p-OmpR.



OmpC and OmpF

   OmpC is an E. coli Promoter that is activated by the EnvZ/OmpR signal transduction system. In the presence of phosphorylated OmpR (called p-OmpR), transcription of OmpC is allowed and genes under the control of OmpC are expressed. In wildtype E. coli, when no p-OmpR is present within the cell, OmpR instead regulates the expression of the promoter OmpF.



EnvZ

   The sensory histidine kinase EnvZ is responsible for controlling the function of OmpR, which in turn upregulates either OmpC or OmpF. EnvZ undergoes autophosphorylation. In high osmolarity conditions, this phosphate group is passed to OmpR, generating p-OmpR. The mechanism by which EnvZ responds to osmolarity is unknown. EnvZ is located in the inner membrane of the cell, containing two transmembrane domains, a cytoplasmic domain, and a periplasmic domain.



RFP

   The RFP we use comes from the iGEM registry ( part BBa_E1010). It is native to the coral Discosoma striata. The exctitation wavelength of RFP is 584nm and it has an emission wavelength of 607nm.


Tar

Tar is an MCP (methyl-accepting chemotaxis protein) native to E. coli that responds primarily to aspartate. Tar contains two transmembrane domains, and a periplasmic ligand sensing domain, which recognizes the presence of aspartate via binding to this region.




UTK

Retrieved from "http://2014.igem.org/Team:UTK-Knoxville/Parts"