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Team:Evry/Notebook/Transposons/09.01.14
From 2014.igem.org
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| + | <h1> Purification of pNK2 with Ori muted </h1> | ||
The product of PCR is purified with dialyse during 2h. | The product of PCR is purified with dialyse during 2h. | ||
<br/> | <br/> | ||
| + | <h1> Competent cells </h1> | ||
To do sorbitol 0,20g/L and glycerol 10%. | To do sorbitol 0,20g/L and glycerol 10%. | ||
<br/>Pseudovibrio competent with wash sorbitol and glycerol in M9 medium. | <br/>Pseudovibrio competent with wash sorbitol and glycerol in M9 medium. | ||
Revision as of 17:44, 17 October 2014
Purification of pNK2 with Ori muted
The product of PCR is purified with dialyse during 2h.Competent cells
To do sorbitol 0,20g/L and glycerol 10%.Pseudovibrio competent with wash sorbitol and glycerol in M9 medium. Sep 01
