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Team:UiOslo Norway/Notebook/ProductsUsed
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Revision as of 11:46, 17 October 2014
Products Used
This is a list of the products we have used.
Enzymes
- DpnI – R0176S, NEB
- EcoRI – R0101S, NEB
- SpeI – R0133S, NEB
- PstI – R0140S, NEB
- T4 Ligase – M0202, NEB
- Q5TM High fidelity DNA polymerase ref: M0491S - NEB
- Takara ExTaqTM Hot start Version. REF: RR006A - Clonetech
Primes and oligos
Primes were ordered from http://www.lifetechnologies.com
Sequencing
Sequencing - We used GATC sequencing.
Kits
-
Miniprep - Plasmid purification.
NucleoSpin® Plasmid, Ref: 740588.25 - Macherey Nagel (MN)
Wizard® Plus SV Minipreps DNA Purification System + Vacuum Adapters, Catalog #A1470 - Promega
-
PCR and Gel clean up
Macherey Nagel (MN) - NucleoSpin® Gel and PCR Clean-up. Ref 740609.50
- Midori Green Advanced DNA stain Cat.No MG03 – Nippon genetics Europe GmbH
